Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-TBX1抗体[EPR3289(2)] (ab109313)

Submit a review Q&A (3)References (9)
Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
  • Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
  • Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
  • Anti-TBX1 antibody [EPR3289(2)] (ab109313)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3289(2)] to TBX1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TBX1抗体[EPR3289(2)]
    参阅全部 TBX1 一抗

  • 描述

    兔单克隆抗体[EPR3289(2)] to TBX1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Jurkat, NCCIT, PC-12, F9, C6, Neuro-2a, HEK-293, HeLa cell lysates

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109313于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
说明
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

靶标

  • 功能

    Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.

  • 疾病相关

    Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
    Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
    Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
    Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies.

  • 序列相似性

    Contains 1 T-box DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Brachyury antibody
      • CAFS antibody
      • CTHM antibody
      • DGCR antibody
      • DGS antibody
      • DORV antibody
      • T box antibody
      • T box 1 antibody
      • T box 1 transcription factor antibody
      • T box 1 transcription factor C antibody
      • T box protein 1 antibody
      • T box transcription factor TBX 1 antibody
      • T box transcription factor TBX1 antibody
      • T-box protein 1 antibody
      • T-box transcription factor TBX1 antibody
      • TBX 1 antibody
      • TBX 1C antibody
      • tbx1 antibody
      • TBX1_HUMAN antibody
      • TBX1C antibody
      • Testis specific T box protein antibody
      • Testis-specific T-box protein antibody
      • TGA antibody
      • VCFS antibody
      see all

    图片

    • Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
      Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
      All lanes : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/1000 dilution

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) control cell lysate
      Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) nuclear cell lysate
      Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : Human liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking buffer: 5% NFDM/TBST.

      There is a strong non-specific band in human liver. Human liver is negative control for TBX1 which is consistent with PMID: 28920943.

    • Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
      Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
      All lanes : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 4 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking buffer: 5% NFDM/TBST.

    • Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
      Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313)
      Lane 1 : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution
      Lane 2 : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/5000 dilution

      Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
      Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST.

      Exposure time: 30 seconds (Lane 1) and 180 seconds (Lane 2).

    • Anti-TBX1 antibody [EPR3289(2)] (ab109313)
      Anti-TBX1 antibody [EPR3289(2)] (ab109313)

    文献 (9)

    发表研究结果有使用 ab109313？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab109313 被引用在 9 文献中.

    • Lin QY  et al. VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. Clin Transl Med 11:e374 (2021). PubMed: 33783987
    • Hou L  et al. Dihydromyricetin Ameliorates Inflammation-Induced Insulin Resistance via Phospholipase C-CaMKK-AMPK Signal Pathway. Oxid Med Cell Longev 2021:8542809 (2021). PubMed: 34650665
    • Hou L  et al. Dihydromyricetin resists inflammation-induced muscle atrophy via ryanodine receptor-CaMKK-AMPK signal pathway. J Cell Mol Med 25:9953-9971 (2021). PubMed: 34676967
    • Meyers K  et al. Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice. Sci Rep 10:14589 (2020). PubMed: 32883997
    • Cui J  et al. TBX1 Functions as a Tumor Activator in Prostate Cancer by Promoting Ribosome RNA Gene Transcription. Front Oncol 10:616173 (2020). PubMed: 33575219
    • Li C  et al. MicroRNA-3651 promotes colorectal cancer cell proliferation through directly repressing T-box transcription factor 1. Int J Mol Med 45:956-966 (2020). PubMed: 31922246
    • Velazquez-Villegas LA  et al. TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue. Nat Commun 9:245 (2018). PubMed: 29339725
    • Hou L  et al. Browning of Pig White Preadipocytes by Co-Overexpressing Pig PGC-1a and Mice UCP1. Cell Physiol Biochem 48:556-568 (2018). PubMed: 30021212
    • Yu Y  et al. Smad7 enables STAT3 activation and promotes pluripotency independent of TGF-ß signaling. Proc Natl Acad Sci U S A 114:10113-10118 (2017). PubMed: 28874583

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    Can you provide the immunogen sequence (or immunogen range) of the Anti-TBX1 antibody [EPR3289(2)] (ab109313).

    Read More
    Answer

    This antibody recognizes an epitope within AA 90-120.

    Read More

    Question

    I hope you are very well. We finished the first trial with the Tbx1 and Twist antibodies and unfortunately we where unable to get a good signal in stages 8-12 embryos. We will give it another trial but wanted to ask you again for some help.

    You have the antiobody ab111926, against CYP26C1, ( mmunogen: Synthetic peptide derived from an internal sequence within Human CYP26C1) and wanted to know if you could provide more information regarding the specific sequence and or expected reactivity against the chick protein...Many tahnks and hope to hear from you soon.

    Read More
    Answer

    Thank you for contacting me and for keeping me updated about the results with the Tbx1 and Twist antibodies. I'm sorry to hear that the results are not good at this point, but please let me know if there is anything I can do to help in that situation.

    Regarding the CYP26C1 antibody, I will get in touch with the lab to enquire about possible chick reactivity, as I don't have the immunogen sequence in my notes. I was only able to find a partial sequence for the chick CYP26C1 sequence-

    http://www.uniprot.org/uniprot/F1NZV9

    Do you have access to a full sequence?

    I will be in touch once I have more information from the lab, and I look forward to hearing from you.

    Have a good day.

    Read More

    Question

    Would like to try these antibodies with chick embryonic tissue staining.

    Read More
    Answer

    Thank you for your call today. Please see below for your testing codes, and let me know if you have any questions or need anything else from me.

    DISCOUNT CODES:
    Ab109313: ***
    Ab49254: ***

    Expiration date: August 28, 2012.

    These codes will eachgive you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in IF with chick samples,and include the correspondingcode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com