AB18530

Anti-TBX1抗体

Name: TBX1抗体 (ab18530)| Abcam中文官网
Brand: Abcam Limited
SKU: AB18530
Rating: 5 (4 reviews)

Anti-TBX1 antibody

(4 Reviews)

(24 Publications)

Rabbit Polyclonal TBX1 antibody. Suitable for WB and reacts with Human samples. Cited in 24 publications.

查看别名

T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein, TBX1

1 Images

Lab

Western blot - Anti-TBX1 antibody (AB18530)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18530 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-TBX1 antibody (ab18530) at 1 µg/mL

Lane 1:

Human heart tissue lysate (total protein) at 20 µg

Lane 2:

Human skeletal muscle tissue lysate (total protein) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 43 kDa

Observed band size: 51 kDa

true

Exposure time: 8min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TBX1 also known as T-box protein 1 is a transcription factor with a molecular weight of around 58 kDa. It belongs to the T-box gene family and plays a role in regulating transcriptional activity of specific genes. TBX1 expresses mostly in mesodermal and neural crest cell derivatives during embryonic development. High expression levels occur in the pharyngeal arches which are structures important for the development of facial and neck regions.

Biological function summary

This protein influences developmental processes involving tissues derived from the pharyngeal arches. TBX1 functions as a part of a regulatory network important for proper embryogenesis. It does not form part of a stable complex but interacts with many cofactors to direct the transcription of genes responsible for cell proliferation differentiation and migration. The correct functioning of TBX1 ensures normal formation and patterning of the craniofacial structures cardiac outflow tract and great vessels.

Pathways

TBX1 engages in the retinoic acid signaling pathway which is essential for the regulation of gene expression during development. It also plays an important role in the fibroblast growth factor (FGF) signaling pathway. These pathways involve related proteins like FGF8 and FGF10 which are important for coordinated signaling during morphogenesis. TBX1's regulatory influence on these pathways highlights its importance in developmental signaling networks.

TBX1 is most notably associated with DiGeorge syndrome a condition resulting from deletions on chromosome 22q11.2 which impacts multiple systems including the heart and immune system. Mutations or deletions affecting TBX1 are linked with the syndrome's characteristic features such as heart defects and craniofacial abnormalities. Additionally disruptions in TBX1 may connect to congenital heart disease interacting with other proteins similarly implicated in structural heart defects during embryonic development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor that plays a key role in cardiovascular development by promoting pharyngeal arch segmentation during embryonic development (By similarity). Also involved in craniofacial muscle development (By similarity). Together with NKX2-5, acts as a regulator of asymmetric cardiac morphogenesis by promoting expression of PITX2 (By similarity). Acts upstream of TBX1 for the formation of the thymus and parathyroid glands from the third pharyngeal pouch (By similarity). Required for hair follicle stem cell self-renewal (By similarity). Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence (PubMed : 11111039, PubMed : 22095455).

See full target information TBX1

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文献 (24)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular research 119:1202-1217 PubMed36635482

2023

STRA6 is essential for induction of vascular smooth muscle lineages in human embryonic cardiac outflow tract development.

Applications

Unspecified application

Species

Unspecified reactive species

Chikai Zhou,Timm Häneke,Eduarde Rohner,Jesper Sohlmér,Polina Kameneva,Artem Artemov,Igor Adameyko,Makoto Sahara

Disease models & mechanisms 15: PubMed35946435

2022

A phenotypic rescue approach identifies lineage regionalization defects in a mouse model of DiGeorge syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella Lania,Monica Franzese,Noritaka Adachi,Marchesa Bilio,Gemma Flore,Annalaura Russo,Erika D'Agostino,Claudia Angelini,Robert G Kelly,Antonio Baldini

Orphanet journal of rare diseases 16:334 PubMed34332615

2021

Variants in a cis-regulatory element of TBX1 in conotruncal heart defect patients impair GATA6-mediated transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Xuechao Jiang,Tingting Li,Sijie Liu,Qihua Fu,Fen Li,Sun Chen,Kun Sun,Rang Xu,Yuejuan Xu

Endocrine-related cancer 28:111-134 PubMed33290252

2020

Gene expression profile in metastatic and non-metastatic parathyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Condello,Filomena Cetani,Maria Denaro,Liborio Torregrossa,Elena Pardi,Paolo Piaggi,Simona Borsari,Anello Marcello Poma,Lucia Anna Muscarella,Paolo Graziano,Maria Grazia Chiofalo,Andrea Repaci,Giovanni Tallini,Francesco Boi,Gabriele Materazzi,Fulvio Basolo,Claudio Marcocci

International journal of molecular sciences 21: PubMed31963474

2020

TBX1 and Basal Cell Carcinoma: Expression and Interactions with and Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Cinzia Caprio,Silvia Varricchio,Marchesa Bilio,Federica Feo,Rosa Ferrentino,Daniela Russo,Stefania Staibano,Daniela Alfano,Caterina Missero,Gennaro Ilardi,Antonio Baldini

Human molecular genetics 28:2295-2308 PubMed31180501

2019

Tbx1 regulates extracellular matrix-cell interactions in the second heart field.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Alfano,Alessandra Altomonte,Claudio Cortes,Marchesa Bilio,Robert G Kelly,Antonio Baldini

Medicines (Basel, Switzerland) 6: PubMed30709026

2019

Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Colette N Miller,Janaiya S Samuels,Yusra Azhar,Ashish Parmar,Rangaiah Shashidharamurthy,Srujana Rayalam

Journal of translational medicine 16:260 PubMed30241482

2018

A loss-of-function mutation p.T52S in RIPPLY3 is a potential predisposing genetic risk factor for Chinese Han conotruncal heart defect patients without the 22q11.2 deletion/duplication.

Applications

Unspecified application

Species

Unspecified reactive species

Nanchao Hong,Erge Zhang,Qingjie Wang,Xiaoqing Zhang,Fen Li,Qihua Fu,Rang Xu,Yu Yu,Sun Chen,Yuejuan Xu,Kun Sun

Molecular genetics & genomic medicine 6:1001-1009 PubMed30209892

2018

MicroRNA-451a acts as tumor suppressor in cutaneous basal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Sun,Pingdong Jiang

Molecular medicine reports 17:7346-7350 PubMed29568912

2018

Clinical study of prenatal ultrasonography combined with T‑box transcription factor 1 as a biomarker for the diagnosis of congenital heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Bingcheng Guo,Jing Xiao,Long Li,Shuanglong Wang,Lijuan Wang,Shuyong Liu

View all publications

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Anti-TBX1抗体

Anti-TBX1 antibody

Western blot - Anti-TBX1 antibody (AB18530)