Anti-TBR1抗体(ab31940)

Gel type: MOPS

Blocking buffer: 3% milk block

IHC image of TBR1 staining in a section of formalin-fixed paraffin-embedded normal E17 mouse brain performed on a Leica BOND^TM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab31940, 1/2000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab31940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

IHC-P image of TBR1 staining on mouse brain sections using ab31940 at a 1:200 dilution.

The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were blocked using 7.5% goat serum for 2 hours at room temperature. ab31940 was diluted 1:200 using blocking buffer and incubated with the sections for 16 hours at 4°C. The secondary antibody used was Goat polyclonal to anti-rabbit conjugated to Alexa Fluor^® 594 (1:400).

DAPI was used to counterstain nuclei.

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(Image courtesy of Human Protein Atlas)
ab31940 staining TBR1 protein in normal human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab31940 at a 1/25 dilution for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.