AB249686

重组Anti-TBP-1抗体[EPR12153] - BSA and Azide free

Anti-TBP-1 antibody [EPR12153] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TBP-1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.

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TBP1, PSMC3, 26S proteasome regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5, Proteasome 26S subunit ATPase 3, Proteasome subunit P50, Tat-binding protein 1, TBP-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] - BSA and Azide free (AB249686)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] - BSA and Azide free (AB249686)

This data was developed using ab171974, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TBP-1 with ab171974 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] - BSA and Azide free (AB249686)

This data was developed using ab171974, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TBP-1 with ab171974 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-TBP-1 antibody [EPR12153] - BSA and Azide free (AB249686)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TBP-1 antibody [EPR12153] - BSA and Azide free (AB249686)

This data was developed using ab171974, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TBP-1 with ab171974 at 1/100 dilution (red) or a Rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-TBP-1 antibody [EPR12153] - BSA and Azide free (AB249686)

This data was developed using ab171974, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TBP-1 antibody [EPR12153] (<a href='/products/primary-antibodies/tbp-1-antibody-epr12153-ab171974'>ab171974</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Predicted band size: 49 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-TBP-1 antibody [EPR12153]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12153

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab249686 is the carrier-free version of ab171974.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TBP-1 also called Tat-binding protein-1 is a critical player in the regulation of protein degradation. It is a component of the 19S regulatory particle of the 26S proteasome. The molecular mass of TBP-1 is approximately 56 kDa. Expression of TBP-1 is noted in various tissues reflecting its essential role in cellular processes. As a proteasome member it contributes to the recognition and unfolding of ubiquitinated proteins designated for degradation.

Biological function summary

TBP-1 functions as an ATPase in the 26S proteasome complex aiding in the breakdown of proteins. This breakdown is important for maintaining protein homeostasis by removing misfolded or damaged proteins. In the proteasome complex TBP-1 collaborates with other ATPases to control access of substrates to the proteolytic core. This role ensures a regulated protein turnover which is important for cell cycle progression and stress responses.

Pathways

TBP-1 serves a significant role in the ubiquitin-proteasome pathway and the regulation of transcription. In the ubiquitin-proteasome pathway it collaborates with other regulatory subunits to modulate proteasomal activity. TBP-1 is associated with proteins like Rpt5 and Rpt1 which are also ATPases in the 19S regulatory particle coordinating the denaturation and translocation of proteins into the 20S core particle for degradation.

TBP-1's malfunction or altered expression can contribute to neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. In these conditions improper protein degradation leads to accumulation of toxic protein aggregates. Through these pathways TBP-1 interacts with proteins like alpha-synuclein in Parkinson's disease where its dysfunction exacerbates aggregation. Understanding TBP-1's role offers insights for therapeutic approaches aimed at modulating the proteasomal degradation machinery in such diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

See full target information PSMC3

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Abcam Product Promise

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重组Anti-TBP-1抗体[EPR12153] - BSA and Azide free