Anti-TBC1D2抗体- N-terminal (ab176701)
Key features and details
- Rabbit polyclonal to TBC1D2 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-TBC1D2抗体- N-terminal
参阅全部 TBC1D2 一抗 -
描述
兔多克隆抗体to TBC1D2 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human TBC1D2 aa 1-50 (N terminal). The exact sequence is proprietary.
Sequence:MEGAGENAPESSSSAPGSEESARDPQVPPPEEESGDCARSLEAVPKKLCG
Database link: Q9BYX2 -
阳性对照
- Recombinant Human TBC1D2 protein (ab163081) can be used as a positive control in WB. HeLa, 293T and Jurkat whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176701于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 105 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
-
相关性
Acts as GTPase-activating protein for RAB7A. Signal effector acting as a linker between RAC1 and RAB7A, leading to RAB7A inactivation and subsequent inhibition of cadherin degradation and reduced cell-cell adhesion. Interacts with activated RAC1 and CDH1. -
细胞定位
Cytoplasm. Cytoplasmic vesicle. Cell junction -
数据库链接
- Entrez Gene: 55357 Human
- Entrez Gene: 609871 Human
- Omim: 609871 Human
- SwissProt: Q9BYX2 Human
-
别名
- PARIS 1 antibody
- PARIS1 antibody
- PP8997 antibody
see all
图片
-
Western blot - Anti-TBC1D2 antibody - N-terminal (ab176701)All lanes : Anti-TBC1D2 antibody - N-terminal (ab176701) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-TBC1D2 antibody - N-terminal (ab176701)Detection of TBC1D2 by Western Blot of Immunprecipitate.
Western blot using ab176701 at 0.4 µg/ml staining TBC1D2 in HeLa whole cell lysate immunoprecipitated using ab176701 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure times of 3 seconds
数据表及文件
-
Datasheet download
文献 (0)
ab176701 尚未被引用在任何文献中。
