AB92627

重组Anti-Tau (phospho S262 + T263)抗体[EPR2454]

Anti-Tau (phospho S262 + T263) antibody [EPR2454]

20ul selling size
RabMAb
Recombinant
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(13 Publications)

Rabbit Recombinant Monoclonal TAU phospho S262 + T263 antibody. Suitable for ELISA, Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 13 publications.

查看别名

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

3 Images

Unknown

Western blot - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (AB92627)

All lanes:

Western blot - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627) at 1/2000 dilution

Lane 1:

SH-SY5Y cell lysate treated with Olcadaic acid & Calyculin A

Lane 2:

SH-SY5Y cell lysate untreated

Predicted band size: 78 kDa

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ELISA - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (AB92627)

ELISA

Unknown

ELISA - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (AB92627)

Serially diluted ab92627 was bound to immobilised phospho S262, phospho T263 or non phospho Tau peptide (100 ng/mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97051; diluted at 1 : 100,000) and signal was developed with ECL substrate.

Unknown

Dot Blot - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (AB92627)

Dot blot analysis of Tau (pS262) peptide (Lane 1), Tau (pT263) peptide (Lane 2) and Tau non-phospho peptide (Lane 3) labelling Tau (pS262 + pT263) with ab92627 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100,000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

不同偶联物与剂型 (1)

Carrier free

Anti-Tau (phospho S262 + T263) antibody [EPR2454] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2454

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ELISA, Dot, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

WB band is consistent with what is found in PMID 16682510 (Peng et. al.)
The specificity of this antibody refers to P10636-8.

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反应性数据

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho S262 + T263

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's research & therapy 17:24 PubMed39827356

2025

Targeting early tau pathology: probiotic diet enhances cognitive function and reduces inflammation in a preclinical Alzheimer's model.

Applications

Unspecified application

Species

Unspecified reactive species

Cassandra M Flynn,Tamunotonye Omoluabi,Alyssa M Janes,Emma J Rodgers,Sarah E Torraville,Brenda L Negandhi,Timothy E Nobel,Shyamchand Mayengbam,Qi Yuan

Aging cell 24:e14405 PubMed39520141

2024

Locus coeruleus vulnerability to tau hyperphosphorylation in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Tamunotonye Omoluabi,Zia Hasan,Jessie E Piche,Abeni R S Flynn,Jules J E Doré,Susan G Walling,Andrew C W Weeks,Touati Benoukraf,Qi Yuan

Journal of neuroinflammation 21:156 PubMed38872143

2024

Profiling the neuroimmune cascade in 3xTg-AD mice exposed to successive mild traumatic brain injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa F Pybus,Sara Bitarafan,Rowan O Brothers,Alivia Rohrer,Arushi Khaitan,Felix Rivera Moctezuma,Kareena Udeshi,Brae Davies,Sydney Triplett,Martin N Griffin,Eric B Dammer,Srikant Rangaraju,Erin M Buckley,Levi B Wood

Brain communications 6:fcae096 PubMed38562310

2024

Hippocampal hyperphosphorylated tau-induced deficiency is rescued by L-type calcium channel blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea A Crossley,Tamunotonye Omoluabi,Sarah E Torraville,Sarah Duraid,Aida Maziar,Zia Hasan,Vishaal Rajani,Kanae Ando,Johannes W Hell,Qi Yuan

Genes to cells : devoted to molecular & cellular mechanisms 29:337-346 PubMed38329182

2024

Microtubule-affinity regulating kinase family members distinctively affect tau phosphorylation and promote its toxicity in a Drosophila model.

Applications

Unspecified application

Species

Unspecified reactive species

Grigorii Sultanakhmetov,Iori Kato,Akiko Asada,Taro Saito,Kanae Ando

Aging 12:6880-6890 PubMed32291379

2020

PP2A subunit PPP2R2C is downregulated in the brains of Alzheimer's transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Waiian Leong,Wei Xu,Bo Wang,Shuaiyun Gao,Xiuyun Zhai,Cuicui Wang,Eric Gilson,Jing Ye,Yiming Lu

PloS one 14:e0225727 PubMed31794565

2019

The KLDpT activation loop motif is critical for MARK kinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Sonntag,James J Moresco,John R Yates,Marc Montminy

Neurobiology of aging 71:255-264 PubMed30172839

2018

S6K/p70S6K1 protects against tau-mediated neurodegeneration by decreasing the level of tau phosphorylated at Ser262 in a Drosophila model of tauopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoki Chiku,Motoki Hayashishita,Taro Saito,Mikiko Oka,Kanako Shinno,Yosuke Ohtake,Sawako Shimizu,Akiko Asada,Shin-Ichi Hisanaga,Koichi M Iijima,Kanae Ando

Biology open 6:1013-1023 PubMed28711868

2017

Human TTBK1, TTBK2 and MARK1 kinase toxicity in is exacerbated by co-expression of human Tau.

Applications

Species

Unspecified reactive species

Josefin Fernius,Annika Starkenberg,Malgorzata Pokrzywa,Stefan Thor

BMC complementary and alternative medicine 17:346 PubMed28668079

2017

Effects of Bu Shen Yi sui capsule on NogoA/NgR and its signaling pathways RhoA/ROCK in mice with experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Fang,Yongqiang Wang,Qi Zheng,Tao Yang,Peiyuan Zhao,Hui Zhao,Qiuxia Zhang,Yuanyuan Zhao,Fang Qi,Kangning Li,Zhenzhen Chen,Junling Li,Nan Zhang,Yongping Fan,Lei Wang

View all publications

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