重组Anti-Tau抗体[EPR22524-95] -小鼠IgG1 (Chimeric) (ab317841)
![Western blot - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-542386-anti-tau-antibody-epr22524-95-western-blot-human-cerebellum-tissue-lysate20-ug.jpg "Western blot - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR22524-95] to Tau - Chimeric
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Tau抗体[EPR22524-95] -小鼠IgG1 (Chimeric)
参阅全部 Tau 一抗 -
描述
小鼠单克隆抗体[EPR22524-95] to Tau - Chimeric -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IHC-Fr or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human cerebellum, mouse brain and rat brain tissue lysate. IHC-P: Human cerebrum, mouse cerebrum, rat cerebrum, mouse hippocampus and rat hippocampus tissue.
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常规说明
This Mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab254256). By design, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22524-95 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317841于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 50-70 kDa (predicted molecular weight: 78 kDa).
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| IHC-P |
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 50-70 kDa (predicted molecular weight: 78 kDa). |
|
IHC-P
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. -
组织特异性
Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. -
疾病相关
Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613. -
序列相似性
Contains 4 Tau/MAP repeats. -
发展阶段
Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. -
结构域
The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats. -
翻译后修饰
Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD. -
细胞定位
Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. - Information by UniProt
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数据库链接
- Entrez Gene: 4137 Human
- Entrez Gene: 17762 Mouse
- Entrez Gene: 29477 Rat
- Omim: 157140 Human
- SwissProt: P10636 Human
- SwissProt: P10637 Mouse
- SwissProt: P19332 Rat
- Unigene: 101174 Human
see all -
形式
There are 9 isoforms produced by alternative splicing. -
别名
- AI413597 antibody
- AW045860 antibody
- DDPAC antibody
see all
图片
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Western blot - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)All lanes : Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human kidney tissue lysate
Lane 4 : Human spleen tissue lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse heart tissue lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Mouse spleen tissue lysate
Lane 9 : Rat brain tissue lysate
Lane 10 : Rat heart tissue lysate
Lane 11 : Rat kidney tissue lysate
Lane 12 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 50-70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver, heart, kidney, spleen (PMID: 24386422).
The identity of the lower band at approximately 15 kDa (in lane 1) is unknown.
Lanes 1-3 blot were developed using a high sensitivity ECL substrate.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-4: 169 seconds; Lanes 5-12: 48 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-2-anti-tau-antibody-epr22524-95-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebral cortex.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-3-anti-tau-antibody-epr22524-95-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebral cortex.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-4-anti-tau-antibody-epr22524-95-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebral cortex.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-5-anti-tau-antibody-epr22524-95-immunohistochemistry-mouse-hippocampus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse hippocampus.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-6-anti-tau-antibody-epr22524-95-immunohistochemistry-rat-hippocampus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat hippocampus.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-7-anti-tau-antibody-epr22524-95-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-8-anti-tau-antibody-epr22524-95-immunohistochemistry-mouse-lliver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Mouse lliver tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-9-anti-tau-antibody-epr22524-95-immunohistochemistry-rat-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Tau with ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat liver.
The section was incubated with ab317841 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)](https://www.abcam.cn/ps/products/317/ab317841/Images/ab317841-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (ab317841)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317841 尚未被引用在任何文献中。