AB75714

Anti-Tau 抗体

Name: Tau抗体 (ab75714)| Abcam中文官网
Brand: Abcam Limited
SKU: AB75714
Price: 4941 CNY
Availability: InStock
Rating: 4 (5 reviews)

Anti-Tau antibody

BOND RX™ Validated
了解详情

(5 Reviews)

(37 Publications)

Chicken Polyclonal TAU antibody. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT conjugated to Keyhole Limpet Haemocyanin.

查看别名

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Big tau

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody (AB75714)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody (AB75714)

Immunohistochemistry (10% Formalin-fixed paraffin-embedded sections) analysis of Human Alzheimer's disease brain (CA1 region of hippocampus) tissue labelling Tau (brown) with ab75714.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody (AB75714)

IHC image of Tau staining in Human Hippocampus (Alzheimer's) (ab4583) formalin-fixed paraffin-embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75714, 5 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

关键信息

宿主种属

Chicken

克隆

Polyclonal

亚型

IgY

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

WB, ICC/IF, ICC, IHC-P

applications

免疫原

Synthetic Peptide within Human MAPT conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10636

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反应性数据

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产品详情

Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 μg/ml, making the total antibody concentration 200 μg/ml.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

IgY fractions purified from immune egg yolks then affinity purified using a peptide column. Equal volumes of both affinity purified anti peptide antibodies were mixed, and the preparation was filter sterilized.

存储溶液

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT

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文献 (37)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113401 PubMed40970196

2025

Mesenchymal stromal cell secretome-induced synaptogenesis is mediated by thrombospondin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Diogo Tomé,Luís F Martins,Miguel Aroso,Henrique Santos,Rui O Costa,João L Afonso,Sofia C Serra,Paulo Aguiar,Carlos B Duarte,João Peça,Paulo S Pinheiro,António J Salgado,Ramiro D Almeida

Signal transduction and targeted therapy 10:295 PubMed40931022

2025

Astrocytic monoamine oxidase B (MAOB)-gamma-aminobutyric acid (GABA) axis as a molecular brake on repair following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Yeong Lee,Jung Moo Lee,Hye-Lan Lee,Jiyeon Park,Heeyoung An,Eun Kyung Park,Sae Yeon Hwang,Sol Lip Yoon,Gwang Yong Hwang,Keung Nyun Kim,Min-Ho Nam,Seung Eun Lee,Hyunji Kang,Joungha Won,Bo Ko Jang,Elijah Hwejin Lee,SunYeong Choi,Mingu Gordon Park,Sang Wook Kim,Ki Duk Park,SeungHwan Lee,C Justin Lee,Yoon Ha

eLife 13: PubMed40762561

2025

The novel role of Kallistatin in linking metabolic syndromes and cognitive memory deterioration by inducing amyloid-β plaques accumulation and tau protein hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Qi,Yanlan Long,Ziming Li,Zhen Zhao,Jinhui Shi,Wanting Xie,Laijian Wang,Yandan Tan,Ti Zhou,Minting Liang,Ping Jiang,Bin Jiang,Xia Yang,Guoquan Gao

Acta neuropathologica communications 13:53 PubMed40057802

2025

Correlative light and electron microscopy imaging of proteinaceous deposits in cell cultures and brain tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Peizhou Jiang,Dennis W Dickson

eLife 13: PubMed39302290

2024

Tumor-infiltrating nerves functionally alter brain circuits and modulate behavior in a mouse model of head-and-neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Barr,Austin Walz,Anthony C Restaino,Moran Amit,Sarah M Barclay,Elisabeth G Vichaya,William C Spanos,Robert Dantzer,Sebastien Talbot,Paola D Vermeer

BMC biology 22:48 PubMed38413974

2024

Primary cilia promote the differentiation of human neurons through the WNT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Coschiera,Masahito Yoshihara,Gilbert Lauter,Sini Ezer,Mariangela Pucci,Haonan Li,Alan Kavšek,Christian G Riedel,Juha Kere,Peter Swoboda

iScience 27:108933 PubMed38318354

2024

Cullin-RING E3 ubiquitin ligase 4 regulates neurite morphogenesis during neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Tammy Shim,Jae Yeon Kim,WonCheol Kim,Yun-Il Lee,Bongki Cho,Cheil Moon

Nature communications 14:8420 PubMed38110419

2023

Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefan Stephen Chen,Mingxi Ou,Stephanie Taylor,Ruxandra Dafinca,Shaohong Isaac Peng,Kevin Talbot,Ho Yin Edwin Chan

International journal of molecular sciences 24: PubMed36834781

2023

Phospholipase D1 Attenuation Therapeutics Promotes Resilience against Synaptotoxicity in 12-Month-Old 3xTg-AD Mouse Model of Progressive Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Chandramouli Natarajan,Charles Cook,Karthik Ramaswamy,Balaji Krishnan

Analytical chemistry 94:9297-9305 PubMed35696262

2022

Simultaneous Multiplexed Imaging of Biomolecules in Transgenic Mouse Brain Tissues Using Mass Spectrometry Imaging: A Multi-omic Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Minh-Uyen Thi Le,Hyun Kyong Shon,Hong-Phuong Nguyen,Chul-Ho Lee,Kyoung-Shim Kim,Hee-Kyung Na,Tae Geol Lee

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