JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB28175

Anti-TATA binding蛋白TBP抗体

Anti-TATA binding protein TBP antibody

4

(3 Reviews)

|

(22 Publications)

Rabbit Polyclonal TATA binding protein TBP antibody. Suitable for ChIP and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TBP.

查看别名

GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

2 Images
ChIP - Anti-TATA binding protein TBP antibody (AB28175)
  • ChIP

Unknown

ChIP - Anti-TATA binding protein TBP antibody (AB28175)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 8μg of ab28175 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

ChIP - Anti-TATA binding protein TBP antibody (AB28175)
  • ChIP

AbReview32840****

ChIP - Anti-TATA binding protein TBP antibody (AB28175)

ChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.
Negative Control : beads.

This image is courtesy of an anonymous Abreview

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ChIP

applications

免疫原

Recombinant Full Length Protein corresponding to Human TBP.

P20226

特异性

Ab28175 recognises TATA binding protein TBP.

style
left-column

替代产品

Primary Antibodies

AB220788

Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade

20ul selling size|Recombinant|Advanced Validation|RabMAb|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade (AB220788)

  • 0
  • 0
Primary Antibodies

AB63766

Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (AB63766)

  • 5
  • 7
Primary Antibodies

AB237785

Anti-TATA binding protein TBP antibody [EPR21954] - ChIP Grade - BSA and Azide free

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - ChIP Grade - BSA and Azide free (AB237785)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB16048

Anti-Lamin B1 antibody - Nuclear Envelope Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB16048)

  • 5
  • 110
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB5131

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

  • 4
  • 48
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5-10 µg for 25 µg chromatin", "ChIP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.9 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.
Biological function summary

TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.

Pathways

TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.

TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 27007846, PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed : 33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473, PubMed : 9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed : 33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed : 26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed : 15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed : 15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed : 15970593).
See full target information TBP
style
left-column

文献 (22)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0306633 PubMed39208271

2024

Taenia solium TAF6 and TAF9 bind to a downstream promoter element present in the Tstbp1 gene core promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Rodríguez-Lima,Ponciano García-Gutiérrez,Lucía Jiménez,Laura A Velázquez-Villegas,Angel Zarain-Herzberg,Roberto Lazzarini,Karel Estrada,Abraham Landa

Science advances 10:eadl4893 PubMed39121214

2024

3D chromatin architecture, BRD4, and Mediator have distinct roles in regulating genome-wide transcriptional bursting and gene network.

Applications

Unspecified application

Species

Unspecified reactive species

Pawel Trzaskoma,SeolKyoung Jung,Aleksandra Pękowska,Christopher H Bohrer,Xiang Wang,Faiza Naz,Stefania Dell'Orso,Wendy D Dubois,Ana Olivera,Supriya V Vartak,Yongbing Zhao,Subhashree Nayak,Andrew Overmiller,Maria I Morasso,Vittorio Sartorelli,Daniel R Larson,Carson C Chow,Rafael Casellas,John J O'Shea

Heliyon 8:e11656 PubMed36458309

2022

PEITC: A resounding molecule averts metastasis in breast cancer cells by regulating PKCδ/Aurora A interplay.

Applications

Unspecified application

Species

Unspecified reactive species

Souvick Biswas,Elizabeth Mahapatra,Salini Das,Madhumita Roy,Sutapa Mukherjee

Human molecular genetics 32:104-121 PubMed35925837

2022

TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wang,Qiyue Chen,Xin Wang,Shasha Zhao,Huan Deng,Baoqiang Guo,Cheng Zhang,Xiaoye Song,Wensheng Deng,Tongcun Zhang,Hongwei Ni

International journal of molecular sciences 22: PubMed34884937

2021

Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shen-Hsing Hsu,Li-Fang Chou,Chung-Hung Hong,Ming-Yang Chang,Chung-Ying Tsai,Ya-Chung Tian,Huang-Yu Yang,Chih-Wei Yang

Nature structural & molecular biology 28:435-442 PubMed33972784

2021

A dual role for H2A.Z.1 in modulating the dynamics of RNA polymerase II initiation and elongation.

Applications

Unspecified application

Species

Unspecified reactive species

Constantine Mylonas,Choongman Lee,Alexander L Auld,Ibrahim I Cisse,Laurie A Boyer

Oncology reports 45:706-716 PubMed33416185

2021

miRNA‑490‑3p promotes the metastatic progression of invasive ductal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Lu,Mei Zhang,Lu Lu,Yan-Zhao Liu,Hai-Hong Zhang,Xiao-Dong Liu

The Journal of biological chemistry 295:8887-8900 PubMed32434928

2020

The transcriptional corepressor CBFA2T3 inhibits all--retinoic acid-induced myeloid gene expression and differentiation in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Nickolas Steinauer,Chun Guo,Jinsong Zhang

International journal of molecular sciences 20: PubMed31277458

2019

The TAFs of TFIID Bind and Rearrange the Topology of the TATA-Less Promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah N Le,Christopher R Brown,Stacy Harvey,Hinrich Boeger,Hans Elmlund,Dominika Elmlund

Molecular oncology 13:1369-1387 PubMed30927552

2019

Promyelocytic leukemia protein (PML) controls breast cancer cell proliferation by modulating Forkhead transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Nikoleta Sachini,Panagiota Arampatzi,Antonios Klonizakis,Christoforos Nikolaou,Takis Makatounakis,Eric W-F Lam,Androniki Kretsovali,Joseph Papamatheakis
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com