AB150412

重组Anti-TARBP1抗体[EPR8727]

Anti-TARBP1 antibody [EPR8727]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal TARBP1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

查看别名

TRM3, TRP185, TARBP1, tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1, TAR RNA-binding protein 1, TAR RNA-binding protein of 185 kDa, TRP-185

1 Images

Unknown

Western blot - Anti-TARBP1 antibody [EPR8727] (AB150412)

All lanes:

Western blot - Anti-TARBP1 antibody [EPR8727] (ab150412) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 182 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-TARBP1 antibody [EPR8727] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8727

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TARBP1 also known as RISC-loading complex subunit TARBP1 weighs approximately 314 amino acids in size. It is widely expressed in various tissues with a significant presence in the brain and other areas with high RNA activity. TARBP1 plays a mechanical role in RNA binding and processing. It influences RNA silencing pathways by interacting with specific RNA molecules which regulate gene expression. This protein modulates post-transcriptional gene silencing by forming complexes with other proteins to assist in targeting RNA molecules for degradation or translational repression.

Biological function summary

TARBP1 is involved in the RNA interference (RNAi) pathway contributing to cellular regulatory mechanisms. It associates closely with the RNA-induced silencing complex (RISC) facilitating the recognition and processing of RNA targets. This protein functions as a cofactor aiding AGO2 in cleaving target mRNA an important part of gene silencing processes within the cellular environment. These interactions are vital for maintaining cellular homeostasis and regulating various gene expression pathways through post-transcriptional modifications.

Pathways

TARBP1 is integral to the RNA-induced silencing complex loading pathway and the microRNA (miRNA) biogenesis pathway. In these pathways TARBP1 works in concert with Dicer a ribonuclease enzyme to facilitate the maturation of miRNAs which are pivotal in gene regulation. This interaction determines the gene expression patterns essential for cellular processes such as development and differentiation highlighting TARBP1’s importance in RNA silencing pathways.

TARBP1 has connections to cancer and neurodegenerative diseases. In cancer altered expression levels of TARBP1 can affect miRNA processing leading to dysregulation of genes involved in cell proliferation and apoptosis contributing to tumorigenesis. Similarly misregulation of miRNA activity influenced by TARBP1 has been observed in Alzheimer's disease where imbalanced RNA processing can lead to neuronal dysfunction. Proteins such as AGO2 and Dicer are intricately connected to these conditions as they collaborate with TARBP1 in the regulation of miRNA pathways influencing disease progression and outcomes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in a subset of tRNA (PubMed : 18412263, PubMed : 31019095, PubMed : 38867004). Selectively mediates Gm18 methylation of tRNAGln-TTG/CTG and tRNASer-TGA/GCT. Gm18 modification can enhance the stability of modified tRNAs (PubMed : 38867004).. (Microbial infection) In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II (PubMed : 7638159, PubMed : 8626763, PubMed : 8846792). Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA (PubMed : 7638159, PubMed : 8626763, PubMed : 8846792).

See full target information TARBP1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 31:1219-1234 PubMed38867004

2024

The tRNA Gm18 methyltransferase TARBP1 promotes hepatocellular carcinoma progression via metabolic reprogramming of glutamine.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Shi,Yangyi Zhang,Yuci Wang,Jie Wang,Yang Gao,Ruiqi Wang,Liyong Wang,Minggang Xiong,Yanlan Cao,Ningjing Ou,Qi Liu,Honghui Ma,Jiabin Cai,Hao Chen

Experimental neurology 360:114293 PubMed36493862

2022

EphA4/EphrinB2 signaling mediates pericyte-induced transient glia limitans formation as a secondary protective barrier after subarachnoid hemorrhage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiru Zhou,Peiwen Guo,Mingxu Duan,Junhan Li,Xufang Ru,Lin Li,Zongduo Guo,John H Zhang,Hua Feng,Yujie Chen,Xiaochuan Sun

RNA (New York, N.Y.) 25:869-880 PubMed31019095

2019

2'--methylation within prokaryotic and eukaryotic tRNA inhibits innate immune activation by endosomal Toll-like receptors but does not affect recognition of whole organisms.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Freund,Daniel K Buhl,Sébastien Boutin,Annika Kotter,Florian Pichot,Virginie Marchand,Tim Vierbuchen,Holger Heine,Yuri Motorin,Mark Helm,Alexander H Dalpke,Tatjana Eigenbrod

View all publications

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重组Anti-TARBP1抗体[EPR8727]