Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free (AB322040)

This data was developed using ab322039, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TAP1 with ab322039 at 1/5000 (0.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human colon.
The section was incubated with ab322039 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free (AB322040)

This data was developed using ab322039, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling TAP1 with ab322039 at 1/5000 (0.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human cervical carcinoma.
The section was incubated with ab322039 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free (AB322040)

This data was developed using ab322039, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Untreated HT-29 (human colorectal adenocarcinoma epithelial cell). (B) HT-29 treated with IFN gamma (100 ng/mL) for 48 hours. tissue labeling TAP1 with ab322039 at 1/8000 (0.062 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nearly no staining in (A) untreated HT-29 positive staining in (B) HT-29 treated with IFN gamma (100 ng/mL) for 48 hours.
The section was incubated with ab322039 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free (AB322040)

This data was developed using ab322039, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TAP1 with ab322039 at 1/5000 (0.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human skin.
The section was incubated with ab322039 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free (AB322040)

This data was developed using ab322039, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling TAP1 with ab322039 at 1/5000 (0.1 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human colon carcinoma.
The section was incubated with ab322039 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free (AB322040)

This data was developed using ab322039, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TAP1 is upregulated in response to IFN gamma treatment.

The identity of the bands between 15 kDa and 40 kDa are unknown.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TAP1 antibody [EPR26236-92] (<a href='/products/primary-antibodies/tap1-antibody-epr26236-92-ab322039'>ab322039</a>) at 1/1000 dilution

Lane 1:

Untreated HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HT-29 treated with 100ng/ml IFN gamma for 48h whole cell lysate at 20 µg

Lane 3:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa treated with 100ng/ml IFN gamma for 48h whole cell lysate at 20 µg

Lane 5:

Human ovary cancer tissue lysate at 20 µg

Lane 6:

Human tonsil tissue lysate at 20 µg

Lane 7:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 68 kDa,36 kDa

false

Exposure time: 1s

• Dot

Supplier Data

Dot Blot - Anti-TAP1 antibody [EPR26236-92] - BSA and Azide free (AB322040)

This data was developed using ab322039, the same antibody clone in a different buffer formulation.

Dot blot analysis of TAP1 using ab322039 at 1 : 1000 (0.498 ug/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 100000 dilution.

Lane1 : His-tagged human TAP1 fragment
Lane2 : His-tagged human TAP2 fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human TAP2.

All lanes:

Dot Blot - Anti-TAP1 antibody [EPR26236-92] (<a href='/products/primary-antibodies/tap1-antibody-epr26236-92-ab322039'>ab322039</a>) at 1/1000 dilution

Lane 1:

His-tagged human TAP1 fragment at 20 ng

Lane 2:

His-tagged human TAP2 fragment at 20 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 48s