Anti-TAOK2 (phospho S181)抗体(ab32591)
Key features and details
- Rabbit polyclonal to TAOK2 (phospho S181)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
-
产品名称
Anti-TAOK2 (phospho S181)抗体
参阅全部 TAOK2 一抗 -
描述
兔多克隆抗体to TAOK2 (phospho S181) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to TAOK2 (phospho S181). Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser181 of TAO2.
Database link: Q9UL54 -
阳性对照
- Rat hippocampal lysates
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32591于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 138 kDa).
|
说明 |
---|
WB
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 138 kDa). |
靶标
-
功能
Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1, but not isoform 2, activates the JNK MAP kinase pathway through the specific activation of the upstream MKK3 and MKK6 kinases. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of IKKA, and thus NF-kappa-B activation, but not that of JNK. Phosphorylates itself, MBP, activated MAPK8 and tubulins. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization (By similarity). Both isoforms are involved in MAPK14 phosphorylation. -
组织特异性
Ubiquitously expressed, with a higher level of expression in testis and brain. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Isoforms 1 and 2 are autophosphorylated.
C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.
isoform 2 is phosphorylated at 'Ser-1031' by MAPK14. This phosphorylation is required PCDH8 for endocytosis. -
细胞定位
Cytoplasmic vesicle membrane. Cytoplasm > cytoskeleton. Nucleus. Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus and Cell projection > dendrite. In dendrites, colocalizes with PCDH8. - Information by UniProt
-
数据库链接
- Entrez Gene: 64666 Rat
- SwissProt: Q9JLS3 Rat
- Unigene: 9575 Rat
-
别名
- 1110033K02Rik antibody
- B230344N16 antibody
- hKFC C antibody
see all
图片
-
Anti-TAOK2 (phospho S181) antibody (ab32591) at 1/1000 dilution + Rat hippocampal lysates
Predicted band size: 138 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab32591 被引用在 1 文献中.
- McNeer JL et al. Arsenic trioxide-dependent activation of thousand-and-one amino acid kinase 2 and transforming growth factor-beta-activated kinase 1. Mol Pharmacol 77:828-35 (2010). WB ; Human . PubMed: 20159944