重组Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181)抗体[EPR4883] (ab124841)
![Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)](https://www.abcam.cn/ps/products/124/ab124841/Images/ab124841-468740-anti-taok2-taok1-taok3-phospho-s177-s181-s181-antibody-epr4883-westernblot-brain-rat.jpg "Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4883] to TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181)抗体[EPR4883]
参阅全部 TAOK2 + TAOK1 + TAOK3 一抗 -
描述
兔单克隆抗体[EPR4883] to TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) -
宿主
Rabbit -
特异性
ab124841 recognizes Human TAOK1 when phosphorylated at Serine 181, TAOK2 when phosphorylated at Serine 181 and TAOK3 when phosphorylated at Serine 177.
ab124841 cross reactivates with MEK1 due to high immunogen homology.
The antibody is detecting TAOK2 + TAOK1 + TAOK3 at around 138 kDa and MAP2K1 at around 44kDa.
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经测试应用
适用于: WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4883 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124841于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 138 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 138 kDa. |
图片
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Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)All lanes : Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution (Purified)
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate, then the membrane treated with Lambda Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 138 kDa
Observed band size: 110-140 kDa why is the actual band size different from the predicted?We are unsure about the nature of the extra bands. Bands at 110 to 140.
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![Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)](https://www.abcam.cn/ps/products/124/ab124841/Images/ab124841-468739-anti-taok2-taok1-taok3-phospho-s177-s181-s181-antibody-epr4883-westernblot-brain-mouse.jpg)
Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)All lanes : Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution (Purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse brain lysate, then the membrane treated with Lambda Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 138 kDa
Observed band size: 110-140 kDa why is the actual band size different from the predicted?We are unsure about the nature of the extra bands. Bands at 110 to 140.
-
![Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)](https://www.abcam.cn/ps/products/124/ab124841/Images/ab124841-468738-anti-taok2-taok1-taok3-phospho-s177-s181-s181-antibody-epr4883-westernblot-hek293-human.jpg)
Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)All lanes : Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 138 kDa
Observed band size: 110-140 kDa why is the actual band size different from the predicted?We are unsure about the nature of other bands. Bands at 110 to 140.
-
![Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)](https://www.abcam.cn/ps/products/124/ab124841/Images/ab124841-3-benefits-of-recombinant-antibodies.png)
Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124841 被引用在 2 文献中.
- Yesilkanal AE et al. Limited inhibition of multiple nodes in a driver network blocks metastasis. Elife 10:N/A (2021). PubMed: 33973518
- Hwang IY et al. Gai Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol 9:687 (2018). PubMed: 29696016