重组Anti-Talin 2抗体[EPR2536(2)] (ab108967)
![Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)](https://www.abcam.cn/ps/products/108/ab108967/Images/ab108967-239850-anti-talin-2-antibody-epr25362-western-blot.jpg "Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2536(2)] to Talin 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
Related conjugates and formulations
概述
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产品名称
Anti-Talin 2抗体[EPR2536(2)]
参阅全部 Talin 2 一抗 -
描述
兔单克隆抗体[EPR2536(2)] to Talin 2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human, African green monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- COS-1, 293T, HeLa, and Jurkat whole cell lysate (ab7899)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2536(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108967于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 270 kDa (predicted molecular weight: 272 kDa).
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 270 kDa (predicted molecular weight: 272 kDa). |
应用说明
Is unsuitable for ICC/IF or IHC-P.
靶标
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功能
As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity. -
序列相似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
细胞定位
Cell junction > focal adhesion. Cell junction > synapse. Cell membrane. Cytoplasm > cytoskeleton. Focal adhesion plaques and synapses. - Information by UniProt
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数据库链接
- Entrez Gene: 83660 Human
- Entrez Gene: 70549 Mouse
- Entrez Gene: 315776 Rat
- Omim: 607349 Human
- SwissProt: Q9Y4G6 Human
- SwissProt: Q71LX4 Mouse
- Unigene: 569438 Human
- Unigene: 610975 Human
see all -
别名
- DKFZp451B1011 antibody
- DKFZp686I0976 antibody
- DKFZp686K0979 antibody
see all
图片
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Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)](https://www.abcam.cn/ps/products/108/ab108967/Images/ab108967-239849-anti-talin-2-antibody-epr25362-western-blot.jpg)
Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + Jurkat cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)](https://www.abcam.cn/ps/products/108/ab108967/Images/ab108967-239848-anti-talin-2-antibody-epr25362-western-blot.jpg)
Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + COS-1 cell lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)](https://www.abcam.cn/ps/products/108/ab108967/Images/ab108967-121459-anti-talin-2-antibody-epr25362-western-blot.jpg)
Western blot - Anti-Talin 2 antibody [EPR2536(2)] (ab108967)All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (unpurified)
Lane 1 : COS-1 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted? -
![Anti-Talin 2 antibody [EPR2536(2)] (ab108967)](https://www.abcam.cn/ps/products/108/ab108967/Images/ab108967-5-benefits-of-recombinant-antibodies.png)
Anti-Talin 2 antibody [EPR2536(2)] (ab108967)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108967 被引用在 3 文献中.
- Wu Y et al. Long Noncoding RNA Lnc-TLN2-4:1 Suppresses Gastric Cancer Metastasis and Is Associated with Patient Survival. J Oncol 2020:8681361 (2020). PubMed: 32256587
- van de Klundert MA et al. Hepatitis B Virus Protein X Induces Degradation of Talin-1. Viruses 8:N/A (2016). WB ; Human . PubMed: 27775586
- Formosa A et al. DNA methylation silences miR-132 in prostate cancer. Oncogene : (2012). PubMed: 22310291