重组Anti-Talin 1抗体[97H6] (ab108480)
![Western blot - Anti-Talin 1 antibody [97H6] (ab108480)](https://www.abcam.cn/ps/products/108/ab108480/Images/ab108480-395343-anti-talin-1-antibody-97h6-western-blot-huvec.jpg "Western blot - Anti-Talin 1 antibody [97H6] (ab108480)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [97H6] to Talin 1
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Talin 1抗体[97H6]
参阅全部 Talin 1 一抗 -
描述
小鼠单克隆抗体[97H6] to Talin 1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HUVEC, HeLa, HepG2, NIH/3T3, MEF and C6 whole cell lysate. IP: NIH/3T3 whole cell lysate. IHC-P: Mouse spleen tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
97H6 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108480于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 0.241 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IP |
Use a concentration of 16.033 µg/ml.
|
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| WB |
Use a concentration of 0.481 µg/ml. Predicted molecular weight: 269 kDa.
|
| 说明 |
|---|
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IHC-P
Use a concentration of 0.241 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
Use a concentration of 16.033 µg/ml. |
|
WB
Use a concentration of 0.481 µg/ml. Predicted molecular weight: 269 kDa. |
靶标
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功能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. -
序列相似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
细胞定位
Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 7094 Human
- Entrez Gene: 21894 Mouse
- Entrez Gene: 313494 Rat
- Omim: 186745 Human
- SwissProt: Q9Y490 Human
- SwissProt: P26039 Mouse
- Unigene: 471014 Human
- Unigene: 208601 Mouse
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别名
- ILWEQ antibody
- Talin 1 antibody
- Talin antibody
see all
图片
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Western blot - Anti-Talin 1 antibody [97H6] (ab108480)All lanes : Anti-Talin 1 antibody [97H6] (ab108480) at 0.481 µg/ml
Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 6 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 269 kDa
Exposure time: 3 secondsThe expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:22306379 and 25751747).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] (ab108480)](https://www.abcam.cn/ps/products/108/ab108480/Images/ab108480-1-anti-talin-1-antibody-97h6-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] (ab108480)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Talin 1 with ab108480 at 1/5000 dilution (0.241µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse spleen. The section was incubated with ab108480 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunoprecipitation - Anti-Talin 1 antibody [97H6] (ab108480)](https://www.abcam.cn/ps/products/108/ab108480/Images/ab108480-2-anti-talin-1-antibody-97h6-immunoprecipitation-nih3t3.jpg)
Immunoprecipitation - Anti-Talin 1 antibody [97H6] (ab108480)Talin 1 was immunoprecipitated from NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab108480 at 1/30 dilution (2µg in 0.35mg lysates) (16.033 µg/ml). Western blot was performed on the immunoprecipitate using ab108480 at 1/1000 dilution (0.481µg/ml). Mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10µg.
Lane 2: ab108480 IP in NIH/3T3 whole cell lysate.
Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab108480 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Anti-Talin 1 antibody [97H6] (ab108480)](https://www.abcam.cn/ps/products/108/ab108480/Images/ab108480-4-benefits-of-recombinant-antibodies.png)
Anti-Talin 1 antibody [97H6] (ab108480)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab108480 被引用在 9 文献中.
- Li C et al. Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer. Theranostics 13:59-76 (2023). PubMed: 36593959
- Neutel CHG et al. The effect of cyclic stretch on aortic viscoelasticity and the putative role of smooth muscle focal adhesion. Front Physiol 14:1218924 (2023). PubMed: 37637147
- Cui Z et al. Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation. Bone Res 10:58 (2022). PubMed: 36031625
- Fang KP et al. Talin-1 correlates with reduced invasion and migration in human hepatocellular carcinoma cells. Asian Pac J Cancer Prev 15:2655-61 (2014). PubMed: 24761880
- Praekelt U et al. New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2. Eur J Cell Biol 91:180-91 (2012). PubMed: 22306379
- Debrand E et al. Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile. Biochem Biophys Res Commun 426:190-5 (2012). PubMed: 22925892
- Monkley SJ et al. Endothelial cell talin1 is essential for embryonic angiogenesis. Dev Biol 349:494-502 (2011). PubMed: 21081121
- Liu J et al. Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis. Mol Cell Biol 31:3366-77 (2011). PubMed: 21670148
- Kopp PM et al. Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1. Eur J Cell Biol 89:661-73 (2010). PubMed: 20605055