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AB109404

重组Anti-TAK1 (phospho S439)抗体[EPR2863]

Anti-TAK1 (phospho S439) antibody [EPR2863]

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(25 Publications)

Rabbit Recombinant Monoclonal TAK1 phospho S439 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 25 publications.

查看别名

TAK1, MAP3K7, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1

4 Images
Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • IP

Unknown

Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

Purified ab109404 at 1/50 dilution (2μg) immunoprecipitating TAK1 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate 10μg
Lane 2 (+) : ab109404 + HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab239974 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 75 kDa

All lanes:

Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

Predicted band size: 67 kDa

Observed band size: 80 kDa

false

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • WB

Unknown

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

All lanes:

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with transforming growth factor-beta (TGF-beta) at 10 µg

Predicted band size: 67 kDa

Observed band size: 75 kDa

false

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • WB

Lab

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

Dilution and blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1β for 10 minutes whole cell lysate at 10 µg

Lane 3:

HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1β for 10 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

Observed band size: 75 kDa

false

Exposure time: 10s

Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)
  • Dot

Lab

Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (AB109404)

Dot Blot analysis of Lane 1 : TAK1 (phospho S439) phospho peptide and Lane 2 : TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time : 3 minutes.

不同偶联物与剂型 (1)

  • Carrier free

    Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2863

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IP, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody only detects TAK1 phosphorylated at Serine 439 in Human or TAK1 phosphorylated at Serine 412 in Mouse.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TAK1 also known as MAP3K7 (Mitogen-activated protein kinase kinase kinase 7) is an important protein kinase that weighs approximately 63 kDa. This protein is expressed in various tissues and cells including HEK 293T cells. TAK1 plays a mechanical role as a part of the MAPK signaling cascade. It phosphorylates and activates downstream kinases which is key to transmitting cellular signals that regulate responses to external stimuli like cytokines and stress.
Biological function summary

TAK1 is involved in several cellular processes necessary for maintaining balance and responding to stress. TAK1 forms a complex with proteins like TAB1 TAB2 and TAB3 which are important for its activation and signaling function. This complex facilitates TAK1's involvement in inflammation and immune response indicating its significance in mediating cellular survival and apoptosis.

Pathways

TAK1 operates within the NF-κB and MAPK pathways two critical routes for cellular response to inflammation and stress. In the NF-κB pathway TAK1 activates IKK which triggers the degradation of IκB freeing NF-κB to move into the nucleus and activate transcription. In the MAPK pathway TAK1 directly influences JNK and p38 cascades revealing regulatory connections to proteins like MEK and ERK.

TAK1 has been linked to conditions such as cancer and inflammatory diseases. In cancers aberrant TAK1 activity can lead to enhanced cell proliferation and survival. Moreover inflammation-related disorders can arise from malfunctioning TAK1 signaling demonstrating its connection to proteins like TNF receptor-associated factors which are involved in inflammatory responses. Understanding TAK1's role in these disorders may pave the way for developing potential therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 10094049, PubMed : 11460167, PubMed : 12589052, PubMed : 16845370, PubMed : 16893890, PubMed : 21512573, PubMed : 8663074, PubMed : 9079627). Plays an important role in the cascades of cellular responses evoked by changes in the environment (PubMed : 10094049, PubMed : 11460167, PubMed : 12589052, PubMed : 16845370, PubMed : 16893890, PubMed : 21512573, PubMed : 8663074, PubMed : 9079627). Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR) (PubMed : 16893890, PubMed : 9079627). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7 (PubMed : 11460167, PubMed : 8663074). These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs); both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1) (PubMed : 11460167, PubMed : 12589052, PubMed : 8663074). Independently of MAP2Ks and p38 MAPKs, acts as a key activator of NF-kappa-B by promoting activation of the I-kappa-B-kinase (IKK) core complex (PubMed : 12589052, PubMed : 8663074). Mechanistically, recruited to polyubiquitin chains of RIPK2 and IKBKG/NEMO via TAB2/MAP3K7IP2 and TAB3/MAP3K7IP3, and catalyzes phosphorylation and activation of IKBKB/IKKB component of the IKK complex, leading to NF-kappa-B activation (PubMed : 10094049, PubMed : 11460167). In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B (PubMed : 16893890). Promotes TRIM5 capsid-specific restriction activity (PubMed : 21512573). Phosphorylates RIPK1 at 'Ser-321' which positively regulates RIPK1 interaction with RIPK3 to promote necroptosis but negatively regulates RIPK1 kinase activity and its interaction with FADD to mediate apoptosis (By similarity). Phosphorylates STING1 in response to cGAMP-activation, promoting association between STEEP1 and STING1 and STING1 translocation to COPII vesicles (PubMed : 37832545).
See full target information MAP3K7 phospho S439
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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:4932970 PubMed40918406

2025

Electroacupuncture Attenuates Hepatic Ischemia-Reperfusion Injury by Modulating the Esr1/TAK1-JNK/p38 Signaling Pathway in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Fan,Wei Guo,Xiaodan Yang,Hao Zhang,Bruno Fink,Lingyu Hu,Xiaoguang Wang

Biology direct 20:73 PubMed40551156

2025

SPI1 upregulated LILRB2 to enhance the immunosuppressive phenotype of LPS-tolerant macrophages by inhibiting TLR8-mediated MyD88/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ruojing Bai,Jun Guo

Nature communications 16:5101 PubMed40456736

2025

Myocardial mitochondrial antiviral signaling protein promotes heart Ischemia-reperfusion injury via RIG-I signaling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Kang,Mengling Yang,Yue Liu,Yang Gui,Yalan Dong,Haifeng Zhou,Zili Zhang,Mingyue Li,Heng Fan,Zheng Li,Junjie Lu,Junyi Li,Rui Zhu,Chengyu Yin,Boyi Liu,Feng Jiang,Kun Huang,Alexey Sarapultsev,Fangfei Li,Ge Zhang,Ling Zhao,Yanyi Wang,Yunjia Ning,Xiang Cheng,Sarajo K Mohanta,Changjun Yin,Shanshan Luo,Andreas J R Habenicht,Desheng Hu

iScience 28:111756 PubMed39925433

2025

Negative regulation of lymphangiogenesis by Tenascin-C delays the resolution of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Katoh,Yoshiyuki Senga,Kento Mizutani,Kazuaki Maruyama,Daishi Yamakawa,Tadashi Yamamuro,Michiaki Hiroe,Keiichi Yamanaka,Akihiro Sudo,Naoyuki Katayama,Toshimichi Yoshida,Kyoko Imanaka-Yoshida

Scientific reports 14:24529 PubMed39424901

2024

Trophoblast cell-derived extracellular vesicles regulate the polarization of decidual macrophages by carrying miR-141-3p in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Dongcai Wu,Bo Zhou,Lan Hong,Hui Cen,Ling Wang,Yanlin Ma,Humin Gong

Frontiers in immunology 15:1386780 PubMed38756773

2024

TAK1 inhibition mitigates intracerebral hemorrhage-induced brain injury through reduction of oxidative stress and neuronal pyroptosis via the NRF2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Chunli Chen,Lite Ge,Zheng Jiang,Zhiping Hu,Lihong Yin

Scientific reports 13:21106 PubMed38036641

2023

Application of human platelet lysate in chondrocyte expansion promotes chondrogenic phenotype and slows senescence progression via BMP-TAK1-p38 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Narong Chitchongyingcharoen,Tulyapruek Tawonsawatruk,Jitrada Phetfong,Wrattya Aroontanee,Aungkura Supokawej

Proceedings of the National Academy of Sciences of the United States of America 119:e2115669119 PubMed35238667

2022

Molecular mechanism underlying the TLR4 antagonistic and antiseptic activities of papiliocin, an insect innate immune response molecule.

Applications

Unspecified application

Species

Unspecified reactive species

Manigandan Krishnan,Joonhyeok Choi,Ahjin Jang,Sungjae Choi,Jiwon Yeon,Mihee Jang,Yeongjoon Lee,Kkabi Son,Soon Young Shin,Myeong Seon Jeong,Yangmee Kim

Communications biology 5:186 PubMed35233032

2022

H5N1 infection impairs the alveolar epithelial barrier through intercellular junction proteins via Itch-mediated proteasomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ruan,Yuling Sun,Jingting Zhang,Jing Sun,Wei Liu,Richard A Prinz,Daxin Peng,Xiufan Liu,Xiulong Xu

Journal of inflammation research 14:2587-2600 PubMed34168482

2021

Genistein Attenuates Isoflurane-Induced Neuroinflammation by Inhibiting TLR4-Mediated Microglial-Polarization in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Jiang,Shoucai Xu,Yangyang Shen,Yong Xu,Yuwen Li
View all publications
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