Anti-TAK1抗体(ab50431)
Key features and details
- Goat polyclonal to TAK1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TAK1抗体
参阅全部 TAK1 一抗 -
描述
山羊多克隆抗体to TAK1 -
宿主
Goat -
特异性
This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Xenopus laevis, Orangutan
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免疫原
Synthetic peptide:
C-AELDQDEKDQQNT
, corresponding to internal sequence amino acids 552-564 of Human TAK1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50431于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 67 kDa).
Primary incubation 1 hour at room temperature. Approx 75kDa band observed in lysates of cell lines NIH/3T3 and A431. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 67 kDa). Primary incubation 1 hour at room temperature. Approx 75kDa band observed in lysates of cell lines NIH/3T3 and A431. |
靶标
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功能
Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP. - Information by UniProt
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数据库链接
- Entrez Gene: 529146 Cow
- Entrez Gene: 6885 Human
- Entrez Gene: 26409 Mouse
- Entrez Gene: 100171454 Orangutan
- Entrez Gene: 313121 Rat
- Omim: 602614 Human
- SwissProt: A2VDU3 Cow
- SwissProt: O43318 Human
see all -
别名
- M3K7_HUMAN antibody
- MAP3K 7 antibody
- Map3k7 antibody
see all
图片
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Western blot - Anti-TAK1 antibody (ab50431)Lanes 1-2 : Anti-TAK1 antibody (ab50431) at 1 µg/ml
Lane 3 : Anti-TAK1 antibody (ab50431) at 2 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Daudi cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 67 kDa -
Western blot - Anti-TAK1 antibody (ab50431)Anti-TAK1 antibody (ab50431) at 0.1 µg/ml + Cell lysates prepared from NIH/3T3 cells at 35 µg
Developed using the ECL technique.
Predicted band size: 67 kDaPrimary antibody incubated for 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab50431 被引用在 1 文献中.
- Shen L et al. A naturally derived small molecule NDSM253 inhibits IKK1 to suppress inflammation response and promote bone healing after fracture. Am J Transl Res 13:24-37 (2021). PubMed: 33527006
