重组Anti-TAB3抗体[EPR5965] (ab124723)
![Western blot - Anti-TAB3 antibody [EPR5965] (ab124723)](https://www.abcam.cn/ps/products/124/ab124723/Images/ab124723-138543-anti-tab3-antibody-epr5965-western-blot.jpg "Western blot - Anti-TAB3 antibody [EPR5965] (ab124723)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5965] to TAB3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-TAB3抗体[EPR5965]
参阅全部 TAB3 一抗 -
描述
兔单克隆抗体[EPR5965] to TAB3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Mouse, Human
不与反应: Rat -
免疫原
Synthetic peptide within Human TAB3 aa 50-150. The exact sequence is proprietary.
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阳性对照
- HepG2 cells; 293T, HepG2, HeLa, Caco-2, RAW264.7 and NIH 3T3 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.20 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5965 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124723于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 79 kDa).
|
|
| ICC/IF |
1/50.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 79 kDa). |
|
ICC/IF
1/50. |
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.
靶标
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功能
Adapter linking MAP3K7/TAK1 and TRAF6 or TRAF2. Mediator of MAP3K7 activation, respectively in the IL1 and TNF signaling pathways. Plays a role in activation of NF-kappa-B and AP1 transcription factor. Isoform 2 may be an oncogenic factor. -
组织特异性
Widely expressed. Constitutively overexpressed in certain tumor tissues. Isoform 1 is a major transcript while isoform 2 is a minor transcript. -
序列相似性
Contains 1 CUE domain.
Contains 1 RanBP2-type zinc finger. -
翻译后修饰
Ubiquitinated; following IL1 stimulation or TRAF6 overexpression. - Information by UniProt
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数据库链接
- Entrez Gene: 257397 Human
- Entrez Gene: 66724 Mouse
- Omim: 300480 Human
- SwissProt: Q8N5C8 Human
- SwissProt: Q571K4 Mouse
- Unigene: 188256 Human
- Unigene: 119646 Mouse
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别名
- MAP3K7IP 3 antibody
- Mitogen-activated protein kinase kinase kinase 7-interacting protein 3 antibody
- NAP1 antibody
see all
图片
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Western blot - Anti-TAB3 antibody [EPR5965] (ab124723)All lanes : Anti-TAB3 antibody [EPR5965] (ab124723) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 79 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-TAB3 antibody [EPR5965] (ab124723)](https://www.abcam.cn/ps/products/124/ab124723/Images/ab124723-138542-anti-tab3-antibody-epr5965-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TAB3 antibody [EPR5965] (ab124723)ab124723, at 1/50 dilution, staining TAB3 in HepG2 cells by Immunofluorescence. -
![OI-RD Scanning - Anti-TAB3 antibody [EPR5965] (ab124723)](https://www.abcam.cn/ps/products/124/ab124723/Images/ab124723-138541-anti-tab3-antibody-epr5965.jpg)
OI-RD Scanning - Anti-TAB3 antibody [EPR5965] (ab124723) -
![Anti-TAB3 antibody [EPR5965] (ab124723)](https://www.abcam.cn/ps/products/124/ab124723/Images/ab124723-4-benefits-of-recombinant-antibodies.png)
Anti-TAB3 antibody [EPR5965] (ab124723)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab124723 被引用在 4 文献中.
- Yuan L et al. EBV infection-induced GPX4 promotes chemoresistance and tumor progression in nasopharyngeal carcinoma. Cell Death Differ N/A:N/A (2022). PubMed: 35105963
- Liu B et al. Downregulation of microRNA-30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients. Mol Med Rep 18:5799-5806 (2018). PubMed: 30365083
- Ren S et al. Hepatitis B Virus Stimulated Fibronectin Facilitates Viral Maintenance and Replication through Two Distinct Mechanisms. PLoS One 11:e0152721 (2016). PubMed: 27023403
- Hu MM et al. TRIM38 inhibits TNFa- and IL-1ß-triggered NF-?B activation by mediating lysosome-dependent degradation of TAB2/3. Proc Natl Acad Sci U S A 111:1509-14 (2014). PubMed: 24434549