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重组RabMAb

重组Anti-TAB2抗体[EPR19670-161] (ab317602)

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Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
  • Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (ab317602)
  • Anti-TAB2 antibody [EPR19670-161] (ab317602)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19670-161] to TAB2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TAB2抗体[EPR19670-161]
    参阅全部 TAB2 一抗

  • 描述

    兔单克隆抗体[EPR19670-161] to TAB2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details
    不适用于: Flow Cyt (Intra),ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell, HeLa whole cell, 293T whole cell, NIH/3T3 whole cell, PC-12 whole cell, THP-1 whole cell, RAW 264.7 whole cell, NR8383 whole cell, Mouse brain tissue and Rat brain tissue lysates. IP: HeLa whole cell lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317602于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Predicted molecular weight: 76 kDa.
IP
1/30.
说明
WB
1/1000. Predicted molecular weight: 76 kDa.
IP
1/30.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

靶标

  • 功能

    Adapter linking MAP3K7/TAK1 and TRAF6. Promotes MAP3K7 activation in the IL1 signaling pathway. The binding of 'Lys-63'-linked polyubiquitin chains to TAB2 promotes autophosphorylation of MAP3K7 at 'Thr-187'. Involved in heart development.

  • 组织特异性

    Widely expressed. In the embryo, expressed in the ventricular trabeculae, endothelial cells of the conotruncal cushions of the outflow tract and in the endothelial cells lining the developing aortic valves.

  • 疾病相关

    Defects in TAB2 are the cause of congenital heart disease non-syndromic type 2 (CHTD2) [MIM:612863]. It is a disease characterized by congenital developmental abnormalities involving structures of the heart. Clinical features include left ventricular outflow tract obstruction, subaortic stenosis, residual aortic regurgitation, atrial fibrillation, bicuspid aortic valve and aortic dilation. Note=A chromosomal aberration involving TAB2 has been found in a family with congenital heart disease. Translocation t(2;6)(q21;q25).

  • 序列相似性

    Contains 1 CUE domain.
    Contains 1 RanBP2-type zinc finger.

  • 翻译后修饰

    Ubiquitinated; following IL1 stimulation or TRAF6 overexpression. Ubiquitination involves RBCK1 leading to proteasomal degradation.
    Phosphorylated.

  • 细胞定位

    Membrane. Cytoplasm > cytosol. Following IL1 stimulation, translocation occurs from the membrane to cytosol.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CHTD2 antibody
    • FLJ21885 antibody
    • KIAA0733 antibody
    • MAP3K7IP2 antibody
    • Mitogen activated protein kinase kinase kinase 7 interacting protein 2 antibody
    • Mitogen-activated protein kinase kinase kinase 7-interacting protein 2 antibody
    • OTTHUMP00000040125 antibody
    • TAB 2 antibody
    • TAB-2 antibody
    • Tab2 antibody
    • TAB2_HUMAN antibody
    • TAK1 binding protein 2 antibody
    • TAK1-binding protein 2 antibody
    • TGF beta activated kinase 1/MAP3K7 binding protein 2 antibody
    • TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 antibody
    • TGF-beta-activated kinase 1-binding protein 2 antibody
    see all

图片

  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
    Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
    All lanes : Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

    Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
    Lane 2 : HeLa transfected with siRNA specifically targeting TAB2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 76 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody is specific to TAB2 and does not cross react with endogenous TAB3.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-TAB3 antibody [EPR5965]  (ab124723) staining at 1/1000 dilution.

  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
    Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
    All lanes : Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

    Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
    Lane 5 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
    Lane 7 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 8 : NR8383 (rat alveolar macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 76 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Jurkat

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    The identity of the lower molecular weight bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-4:  158 seconds; Lane 5-8: 180 seconds.

  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
    Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)
    All lanes : Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 76 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    The identity of the lower molecular weight bands are unknown.

    Exposure time: Lanes 1:  92 seconds; Lane 2: 114 seconds.

  • Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (ab317602)
    Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (ab317602)

    TAB2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317602 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317602 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: ab317602 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317602 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Anti-TAB2 antibody [EPR19670-161] (ab317602)
    Anti-TAB2 antibody [EPR19670-161] (ab317602)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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