重组Anti-T7 tag®抗体[EPR28033-8] - BSA and Azide free (ab317259)
![Western blot - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-539808-anti-t7-tag-antibody-epr28033-8-western-blot-293t-human-embryonic-kidney-epithelial-cell-transfected-with-an-human-histone-h33-expression-vector-20-ug.jpg "Western blot - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28033-8] to T7 tag® - BSA and Azide free
- Suitable for: Indirect ELISA, ICC/IF, WB, IHC-P, IP
- Reacts with: Species independent
Related conjugates and formulations
概述
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产品名称
Anti-T7 tag®抗体[EPR28033-8] - BSA and Azide free
参阅全部 T7 tag® 一抗 -
描述
兔单克隆抗体[EPR28033-8] to T7 tag® - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Indirect ELISA, ICC/IF, WB, IHC-P, IPmore details
不适用于: ChIP-seq or Flow Cyt (Intra) -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 293T and HeLa transfected with an Human CTCF expression vector containing a T7 tag®, whole lysates. IHC-P: Hela transfected with a T7 tag®-CTCF expression vector cell pellet ICC: HeLa cells. IP: HeLa cell.
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常规说明
ab317259 is the carrier-free version of ab317258
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28033-8 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
- Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842)
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317259于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Indirect ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
靶标
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相关性
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
别名
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
图片
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Western blot - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)All lanes : Anti-T7 tag® antibody [EPR28033-8] (ab317258) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with an Human Histone H3.3 expression vector containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) transfected with an Human Histone H3 expression vector containing a T7 tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 15 kDa why is the actual band size different from the predicted?
Exposure time: 55 secondsThis data was developed using ab317258, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
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![Western blot - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-539809-anti-t7-tag-antibody-epr28033-8-western-blot-hela-human-cervix-adenocarcinoma-epithelial-cell-transfected-withempty-vector-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)All lanes : Anti-T7 tag® antibody [EPR28033-8] (ab317258) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with empty vector, whole cell lysate
Lane 2 : HeLa transfected with an Human CTCF expression vector containing a T7 tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Exposure time: 8 secondsThis data was developed using ab317258, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-CTCF antibody [EPR7314 (B)] - ChIP Grade (ab128873) staining at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-2-anti-t7-tag-antibody-epr28033-8-immunohistochemistry-a-hela-human-cerv.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)This data was developed using ab317258, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Hela (human cervix adenocarcinoma epithelial cell) transfected with a T7 tag®-CTCF expression vector cell pellet. (B) Hela transfected with empty vector cell pellet tissue labeling T7 tag® with ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on Hela transfected with a T7 tag®-CTCF expression vector cell pellet (image A). No staining on Hela transfected with empty vector cell pellet (image B).The section was incubated with ab317258 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-3-anti-t7-tag-antibody-epr28033-8-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)This data was developed using ab317258, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling T7 tag® with ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum.The section was incubated with ab317258 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-4-anti-t7-tag-antibody-epr28033-8-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)This data was developed using ab317258, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling T7 tag® with ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cerebrum.The section was incubated with ab317258 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-5-anti-t7-tag-antibody-epr28033-8-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)This data was developed using ab317258, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling T7 tag® with ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cerebrum.The section was incubated with ab317258 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunocytochemistry/ Immunofluorescence - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-1-anti-t7-tag-antibody-epr28033-8-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)This data was developed using ab317258, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) transfected with a T7 tag®-CTCF expression vector cells labelling T7 tag® with ab317258 at 1/1000 (0.527 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing nuclear staining in HeLa cells transfected with a T7 tag®-CTCF expression vector (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
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![Immunoprecipitation - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-6-anti-t7-tag-antibody-epr28033-8-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)This data was developed using ab317258, the same antibody clone in a different buffer formulation.
T7 tag® as immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) transfected with an Human CTCF expression vector containing a T7 tag® whole cell lysate with ab317258 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317258 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with an Human CTCF expression vector containing a T7 tag®, whole cell lysate
Lane 2: ab317258 IP in HeLa (human cervix adenocarcinoma epithelial cell) transfected with an Human CTCF expression vector containing a T7 tag® whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317258 in HeLa transfected with an Human CTCF expression vector containing a T7 tag® whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
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![Indirect ELISA - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-7-anti-t7-tag-antibody-epr28033-8-elisa-t7-tag.jpg)
Indirect ELISA - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)This data was developed using ab317258, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of ab317258 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: T7 tag®.
Antigen concentration: 1000 ng/ml
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![Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)](https://www.abcam.cn/ps/products/317/ab317259/Images/ab317259-8-benefitsofrecombinantantibodies.jpg)
Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
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