Anti-T7 tag®抗体(ab9115)
Key features and details
- Rabbit polyclonal to T7 tag®
- Suitable for: ICC/IF, ELISA, WB
- Reacts with: Species independent
- Isotype: unknown
概述
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产品名称
Anti-T7 tag®抗体
参阅全部 T7 tag® 一抗 -
描述
兔多克隆抗体to T7 tag® -
宿主
Rabbit -
特异性
ELISA: The anti T7 diluted 1:176,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. -
经测试应用
适用于: ICC/IF, ELISA, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to Escherichia phage T7 T7 tag® aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: P19727 -
常规说明
T7-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support. -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
靶标
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相关性
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
别名
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab9115 被引用在 9 文献中.
- Fiesel FC et al. Substitution of PINK1 Gly411 modulates substrate receptivity and turnover. Autophagy 19:1711-1732 (2023). PubMed: 36469690
- Lee H et al. Enhancement of Wound Healing Efficacy by Increasing the Stability and Skin-Penetrating Property of bFGF Using 30Kc19α-Based Fusion Protein. Adv Biol (Weinh) 5:e2000176 (2021). PubMed: 33724733
- Meng F et al. A bHLH transcription activator regulates defense signaling by nucleo-cytosolic trafficking in rice. J Integr Plant Biol 62:1552-1573 (2020). PubMed: 32129570
- Herrmann A et al. The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation. Mob DNA 9:11 (2018). PubMed: 29610582
- Pye H et al. Using antibody directed phototherapy to target oesophageal adenocarcinoma with heterogeneous HER2 expression. Oncotarget 9:22945-22959 (2018). PubMed: 29796164
- Prinz E et al. WDR62 mediates TNFa-dependent JNK activation via TRAF2-MLK3 axis. Mol Biol Cell 29:2470-2480 (2018). PubMed: 30091641
- Ando M et al. The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity. Mol Neurodegener 12:32 (2017). PubMed: 28438176
- Horn AV et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013). ICC/IF ; Human . PubMed: 24101588
- Kubota T et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem 283:25660-70 (2008). WB . PubMed: 18635538