AB137585

Anti-T Plastin/PLS3 抗体

Name: T Plastin/PLS3抗体 (ab137585)| Abcam中文官网
Brand: Abcam Limited
SKU: AB137585
Price: 5249 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-T Plastin/PLS3 antibody

(3 Reviews)

(10 Publications)

Rabbit Polyclonal T Plastin/PLS3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLS3 aa 50-400.

查看别名

Plastin-3, T-fimbrin, T-plastin, PLS3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-T Plastin/PLS3 antibody (AB137585)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-T Plastin/PLS3 antibody (AB137585)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling T-Plastin protein using ab137585 at a 1/100 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/500 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T Plastin/PLS3 antibody (AB137585)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T Plastin/PLS3 antibody (AB137585)

Immunohistochemical analysis of paraffin-embedded mouse cervix tissue stained for T-Plastin protein with ab137585 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.

Unknown

Western blot - Anti-T Plastin/PLS3 antibody (AB137585)

7.5% SDS PAGE

All lanes:

Western blot - Anti-T Plastin/PLS3 antibody (ab137585) at 1/1000 dilution

Lane 1:

NIH-3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Lane 3:

BCL-1 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human PLS3 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

P13797

特异性

ab137585 may also detect Plastin L as the sequence shows 82% identity.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The T Plastin also known as PLS3 is a protein with a mass of around 70 kDa. This protein belongs to the plastin family and another popular alias is plastine. T Plastin participates in cellular mechanics by bundling actin filaments which is essential for maintaining cell structure and motility. It occurs in various tissues but is highly expressed in the intestines testis and other cells with rapid movement needs like immune cells. Its presence supports functions needing dynamic rearrangement of the cytoskeleton.

Biological function summary

T Plastin plays significant roles in several cellular activities. It stabilizes the actin cytoskeleton which impacts cell division signal transduction and cellular interactions. T Plastin is not part of a multi-protein complex but works closely with actin and other actin-binding proteins to regulate cytoskeletal organization. By supporting these functions T Plastin indirectly influences cell shape and intracellular transport mechanisms.

Pathways

T Plastin integrates into the actin dynamics pathways important for cytoskeletal reorganization. It works alongside proteins such as cofilin and profilin which also regulate actin dynamics. Another important pathway includes wound healing where T Plastin's actin-bundling function assists in cell migration and tissue regeneration. In these pathways its role ensures efficient cellular responses to environmental changes.

Defects in T Plastin have been linked to osteoporosis and some forms of cancers. In osteoporosis improper expression of T Plastin affects bone density regulation partly due to its influence on osteoclast function. Cancers such as colorectal cancer exhibit altered T Plastin levels which may impact metastasis due to changes in cell motility. In these contexts T Plastin's interaction with other proteins like integrins highlights its importance in controlling cell adhesion and migration associated with disease progress.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Actin-bundling protein.

See full target information PLS3

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:999 PubMed36890170

2023

Tyrosyl-tRNA synthetase has a noncanonical function in actin bundling.

Applications

Unspecified application

Species

Unspecified reactive species

Biljana Ermanoska,Bob Asselbergh,Laura Morant,Maria-Luise Petrovic-Erfurth,Seyyedmohsen Hosseinibarkooie,Ricardo Leitão-Gonçalves,Leonardo Almeida-Souza,Sven Bervoets,Litao Sun,LaTasha Lee,Derek Atkinson,Akram Khanghahi,Ivaylo Tournev,Patrick Callaerts,Patrik Verstreken,Xiang-Lei Yang,Brunhilde Wirth,Avital A Rodal,Vincent Timmerman,Bruce L Goode,Tanja A Godenschwege,Albena Jordanova

Arteriosclerosis, thrombosis, and vascular biology 40:175-188 PubMed31694393

2019

S-Nitrosylation of Plastin-3 Exacerbates Thoracic Aortic Dissection Formation via Endothelial Barrier Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Pan,Zhe Lin,Xin Tang,Jiaxin Tian,Qiao Zheng,Jin Jing,Liping Xie,Hongshan Chen,Qiulun Lu,Hong Wang,Qingguo Li,Yi Han,Yong Ji

Endocrinology 160:484-503 PubMed30649248

2019

Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wen,Siwen Wu,Will M Lee,Chris K C Wong,Wing-Yee Lui,Bruno Silvestrini,C Yan Cheng

Cell death & disease 9:208 PubMed29434191

2018

Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis.

Applications

Species

Unspecified reactive species

Qing Wen,Nan Li,Xiang Xiao,Wing-Yee Lui,Darren S Chu,Chris K C Wong,Qingquan Lian,Renshan Ge,Will M Lee,Bruno Silvestrini,C Yan Cheng

Scientific reports 7:1110 PubMed28439067

2017

Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2.

Applications

Species

Rat

Ying Gao,Haiqi Chen,Xiang Xiao,Wing-Yee Lui,Will M Lee,Dolores D Mruk,C Yan Cheng

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 31:584-597 PubMed27815338

2016

Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Gao,Dolores Mruk,Haiqi Chen,Wing-Yee Lui,Will M Lee,C Yan Cheng

Scientific reports 6:28589 PubMed27358069

2016

Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Gao,Wing-Yee Lui,Will M Lee,C Yan Cheng

Spermatogenesis 6:e1206353 PubMed27559491

2016

Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Li,Will M Lee,C Yan Cheng

FASEB journal : official publication of the Federa 29:3788-805 PubMed26048141

2015

Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Li,Dolores D Mruk,Chris K C Wong,Will M Lee,Daishu Han,C Yan Cheng

Gastroenterology 146:1386-96.e1-17 PubMed24462734

2014

Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, and is associated with patient outcomes.

Applications

Unspecified application

Species

Mouse

Ang Li,Jennifer P Morton,YaFeng Ma,Saadia A Karim,Yan Zhou,William J Faller,Emma F Woodham,Hayley T Morris,Richard P Stevenson,Amelie Juin,Nigel B Jamieson,Colin J MacKay,C Ross Carter,Hing Y Leung,Shigeko Yamashiro,Karen Blyth,Owen J Sansom,Laura M Machesky

View all publications

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Abcam Product Promise

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Anti-T Plastin/PLS3 抗体

Anti-T Plastin/PLS3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-T Plastin/PLS3 antibody (AB137585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T Plastin/PLS3 antibody (AB137585)

Western blot - Anti-T Plastin/PLS3 antibody (AB137585)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (10)

Abcam Product Promise

Anti-T Plastin/PLS3 抗体

Anti-T Plastin/PLS3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-T Plastin/PLS3 antibody (AB137585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T Plastin/PLS3 antibody (AB137585)

Western blot - Anti-T Plastin/PLS3 antibody (AB137585)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

替代产品

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (10)

Abcam Product Promise