Anti-T-bet / Tbx21抗体(ab53174)
Key features and details
- Rabbit polyclonal to T-bet / Tbx21
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-T-bet / Tbx21抗体
参阅全部 T-bet / Tbx21 一抗 -
描述
兔多克隆抗体to T-bet / Tbx21 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human T-bet/ Tbx21.
参阅Abcam的专有抗源政策 (Peptide available as ab69655.) -
阳性对照
- This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, Jurkat Nuclear, Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM), E14Tg2a Whole Cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
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| 说明 |
|---|
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IP
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa). |
靶标
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功能
Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. -
组织特异性
T-cell specific. -
疾病相关
Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities. -
序列相似性
Contains 1 T-box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 30009 Human
- Entrez Gene: 57765 Mouse
- Omim: 604895 Human
- SwissProt: Q9UL17 Human
- SwissProt: Q9JKD8 Mouse
- Unigene: 272409 Human
- Unigene: 94519 Mouse
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别名
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
see all
图片
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Immunoprecipitation - Anti-T-bet / Tbx21 antibody (ab53174)
T-bet / Tbx21 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53174.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 53kDa; T-bet / Tbx21
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Western blot - Anti-T-bet / Tbx21 antibody (ab53174)All lanes : Anti-T-bet / Tbx21 antibody (ab53174) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab53174 被引用在 3 文献中.
- Yue-Chun L et al. Vagus nerve plays a pivotal role in CD4+ T cell differentiation during CVB3-induced murine acute myocarditis. Virulence 12:360-376 (2021). PubMed: 33380272
- Fu X et al. Staphylococcal Enterotoxin C2 Mutant-Directed Fatty Acid and Mitochondrial Energy Metabolic Programs Regulate CD8+ T Cell Activation. J Immunol 205:2066-2076 (2020). PubMed: 32938730
- Lin B et al. Honeysuckle extract relieves ovalbumin-induced allergic rhinitis by inhibiting AR-induced inflammation and autoimmunity. Biosci Rep 39:N/A (2019). PubMed: 31308153