重组Anti-SYVN1/HRD1抗体[EP7459] (ab170901)
![Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.cn/ps/products/170/ab170901/Images/ab170901-172098-anti-syvn1-antibody-ep7459-western-blot.jpg "Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7459] to SYVN1/HRD1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SYVN1/HRD1抗体[EP7459]
参阅全部 SYVN1/HRD1 一抗 -
描述
兔单克隆抗体[EP7459] to SYVN1/HRD1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: Q86TM6 -
阳性对照
- 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP7459 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170901于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
|
| ICC/IF |
1/100 - 1/250.
|
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
|
ICC/IF
1/100 - 1/250. |
|
IP
1/10 - 1/100. |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. -
组织特异性
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger. -
结构域
The RING-type zinc finger is required for E3 ligase activity. -
翻译后修饰
Not N-glycosylated.
Auto-ubiquitinated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 84447 Human
- Entrez Gene: 74126 Mouse
- Entrez Gene: 361712 Rat
- Omim: 608046 Human
- SwissProt: Q86TM6 Human
- SwissProt: Q9DBY1 Mouse
- Unigene: 75859 Human
- Unigene: 149870 Mouse
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别名
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
see all
图片
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Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)All lanes : Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.cn/ps/products/170/ab170901/Images/ab170901-172100-anti-syvn1-antibody-ep7459-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.cn/ps/products/170/ab170901/Images/ab170901-172099-anti-syvn1-antibody-ep7459-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution
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![Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.cn/ps/products/170/ab170901/Images/ab170901-172097-anti-syvn1-antibody-ep7459-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).
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![Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.cn/ps/products/170/ab170901/Images/ab170901-5-benefits-of-recombinant-antibodies.png)
Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab170901 被引用在 21 文献中.
- Nishiguchi H et al. Luteolin Protects Against 6-Hydoroxydopamine-Induced Cell Death via an Upregulation of HRD1 and SEL1L. Neurochem Res 49:117-128 (2024). PubMed: 37632637
- Xie W et al. SYVN1 ubiquitinates FoxO1 to induce β-catenin nuclear translocation, PD-L1-mediated metastasis, and immune evasion of hepatocellular carcinoma. Cell Oncol (Dordr) 46:1285-1299 (2023). PubMed: 37099251
- Shi Y et al. E3 ubiquitin ligase SYVN1 is a key positive regulator for GSDMD-mediated pyroptosis. Cell Death Dis 13:106 (2022). PubMed: 35115505
- Sun L et al. The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission. Oxid Med Cell Longev 2022:3639302 (2022). PubMed: 36193086
- Ma C et al. miR-602 Activates NRF2 Antioxidant Pathways to Protect HBMECs from OGD/R-Induced Oxidative Stress via Targeting KEAP1 and HRD1. Dis Markers 2022:6967573 (2022). PubMed: 36193504