Anti-SYVN1/HRD1抗体(ab118483)
Key features and details
- Rabbit polyclonal to SYVN1/HRD1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-SYVN1/HRD1抗体
参阅全部 SYVN1/HRD1 一抗 -
描述
兔多克隆抗体to SYVN1/HRD1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human SYVN1/HRD1 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: NP_115807.1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100 - 1/500. Predicted molecular weight: 67 kDa.
|
|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/100 - 1/500. Predicted molecular weight: 67 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. -
组织特异性
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger. -
结构域
The RING-type zinc finger is required for E3 ligase activity. -
翻译后修饰
Not N-glycosylated.
Auto-ubiquitinated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 84447 Human
- Entrez Gene: 74126 Mouse
- Omim: 608046 Human
- SwissProt: Q86TM6 Human
- SwissProt: Q9DBY1 Mouse
- Unigene: 75859 Human
- Unigene: 149870 Mouse
-
别名
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)
ab118483 at 10µg/ml staining SYVN1/HRD1 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)ab118483 at 10µg/ml staining SYVN1/HRD1 in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
Western blot - Anti-SYVN1/HRD1 antibody (ab118483)Anti-SYVN1/HRD1 antibody (ab118483) at 1/100 dilution + T47D cell lysate at 35 µg
Predicted band size: 67 kDa -
Western blot - Anti-SYVN1/HRD1 antibody (ab118483)Anti-SYVN1/HRD1 antibody (ab118483) at 1/100 dilution + Mouse kidney tissue lysate
Predicted band size: 67 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab118483 被引用在 4 文献中.
- Qin H et al. RIOK3 promotes mTORC1 activation by facilitating SLC7A2-mediated arginine uptake in pancreatic ductal adenocarcinoma. Aging (Albany NY) 15:1039-1051 (2023). PubMed: 36880835
- Guo X et al. HRD1 inhibits fatty acid oxidation and tumorigenesis by ubiquitinating CPT2 in triple-negative breast cancer. Mol Oncol 15:642-656 (2021). PubMed: 33207079
- Fan Y et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer 20:25 (2021). PubMed: 33530981
- Cheng X et al. ATRA protects skin fibroblasts against UV-induced oxidative damage through inhibition of E3 ligase Hrd1. Mol Med Rep 20:2294-2302 (2019). PubMed: 31322186