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AB250732

重组Anti-SYPL1抗体[EPR14317] - BSA and Azide free

Anti-SYPL1 antibody [EPR14317] - BSA and Azide free

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Rabbit Recombinant Monoclonal SYPL1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

查看别名

SYPL, SYPL1, Synaptophysin-like protein 1, Pantophysin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)

This data was developed using ab184176, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)

This data was developed using ab184176, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)

This data was developed using ab184176, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling SYPL1 with ab184176 at 1/130 dilution (red) or a rabbit IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)
  • WB

Supplier Data

Western blot - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)

This data was developed using ab184176, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SYPL1 antibody [EPR14317] (<a href='/products/primary-antibodies/sypl1-antibody-epr14317-ab184176'>ab184176</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 20 µg

Lane 2:

Human adipose tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa

false

Western blot - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)
  • WB

Supplier Data

Western blot - Anti-SYPL1 antibody [EPR14317] - BSA and Azide free (AB250732)

This data was developed using ab184176, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SYPL1 antibody [EPR14317] (<a href='/products/primary-antibodies/sypl1-antibody-epr14317-ab184176'>ab184176</a>) at 1/5000 dilution

Lane 1:

Human fetal lung tissue lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14317

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

ab250732 is the carrier-free version of ab184176.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information SYPL1
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搭配使用产品

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BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
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AB18258

Anti-PSD95 antibody - Synaptic Marker

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Western blot - Anti-PSD95 antibody - Synaptic Marker (AB18258)

  • 5
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Primary Antibodies

AB254349

Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

BOND RX™ Validated|Lab Essentials|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB254349)

  • 4
  • 1
Primary Antibodies

AB182744

Anti-Collagen VI antibody [EPR17072]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (AB182744)

  • 5
  • 5

Abcam Product Promise

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详情请参阅我们的条款与条件。

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