AB184545

重组Anti-Syntaxin 4抗体[EPR15473]

Anti-Syntaxin 4 antibody [EPR15473]

RabMAb
Recombinant
KO Validated
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(7 Publications)

Rabbit Recombinant Monoclonal Syntaxin 4 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 7 publications.

查看别名

STX4A, STX4, Syntaxin-4, Renal carcinoma antigen NY-REN-31

3 Images

Lab

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (AB184545)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Syntaxin 4 knockout HAP1 cell lysate (20 μg)
Lane 3 : K562 cell lysate (20 μg)
Lane 4 : HeLa cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab184545 observed at 1X kDa. Red - loading control, ab18058, observed at 124 kDa.

ab184545 was shown to recognize Syntaxin 4 when Syntaxin 4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Syntaxin 4 knockout samples were subjected to SDS-PAGE. ab184545 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)

Predicted band size: 34 kDa

Observed band size: 34 kDa

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Supplier Data

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (AB184545)

All lanes:

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/2000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (AB184545)

All lanes:

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/10000 dilution

All lanes:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15473

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 4 also known as STX4 is a member of the soluble NSF attachment protein receptor (SNARE) family. It has a molecular mass of approximately 33 kDa. Syntaxin 4 is integral to membrane fusion processes specifically docking and fusion of vesicles with target membranes. It expresses in a wide range of tissues but high levels are found in skeletal muscle and adipose tissue. Its function is necessary in cellular trafficking systems and exocytosis.

Biological function summary

Syntaxin 4 facilitates key transport events at the plasma membrane. It forms part of the SNARE complex which is essential for vesicle targeting and fusion. This complex includes other SNARE proteins such as VAMP2 and SNAP23 ensuring that vesicles deliver their content accurately within cells. This function is critical in processes such as insulin-stimulated glucose uptake and neurotransmitter release.

Pathways

Syntaxin 4 involves itself in the regulation of vesicular transport and insulin signaling. It plays an essential role in the GLUT4 translocation pathway where Syntaxin 4 interacts with proteins like Munc18c which is critical for the regulation of glucose transport in response to insulin. Another important pathway includes its involvement in neurotransmitter release where Syntaxin 4 interfaces with synaptotagmin highlighting its influence within signal transmission processes.

Dysfunctions related to Syntaxin 4 have implications in conditions such as type 2 diabetes and certain neuropathies. In the context of type 2 diabetes impaired Syntaxin 4-mediated uptake of glucose into cells can lead to insulin resistance. Syntaxin 4 also relates to neuromuscular disorders where its malfunction can alter neurotransmitter release. These conditions oftentimes involve Munc18c which stabilizes Syntaxin 4 and modulates its activity within the cell.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plasma membrane t-SNARE that mediates docking of transport vesicles (By similarity). Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane (By similarity). In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane (By similarity). Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones (By similarity). Required for normal hearing (PubMed : 36355422).

See full target information STX4

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 26:2089-2103 PubMed35146909

2022

CD44/ERM/F-actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Shao,Linbin Li,Lu Liu,Yunxi Yang,Jiamin Huang,Dongdong Ji,Yuying Zhou,Yi Chen,Zhechen Zhu,Bingwei Sun

Cell reports 32:108107 PubMed32905772

2020

Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoe Kitao,Kyoichiro Taguchi,Shintaro Seto,Kohei Arasaki,Hiroki Ando,Hiroki Nagai,Tomoko Kubori

Brain : a journal of neurology 143:1780-1797 PubMed32428221

2020

Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenwei Yu,Min Shi,Tessandra Stewart,Pierre-Olivier Fernagut,Yang Huang,Chen Tian,Benjamin Dehay,Anzari Atik,Dishun Yang,Francesca De Giorgi,François Ichas,Marie-Hélène Canron,Roberto Ceravolo,Daniela Frosini,Han-Joon Kim,Tao Feng,Wassilios G Meissner,Jing Zhang

Neuron 102:1184-1198.e10 PubMed31072786

2019

Activity-Dependent Secretion of Synaptic Organizer Cbln1 from Lysosomes in Granule Cell Axons.

Applications

Unspecified application

Species

Unspecified reactive species

Keiji Ibata,Maya Kono,Sakae Narumi,Junko Motohashi,Wataru Kakegawa,Kazuhisa Kohda,Michisuke Yuzaki

Frontiers in neuroscience 12:985 PubMed30670942

2019

Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Lizarbe,Ana Francisca Soares,Sara Larsson,João M N Duarte

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen

Journal of virology 91: PubMed28356525

2017

MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2.

Applications

Species

Human

Mitsuhiro Machitani,Fuminori Sakurai,Keisaku Wakabayashi,Kosuke Nakatani,Masashi Tachibana,Hiroyuki Mizuguchi

View all publications

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重组Anti-Syntaxin 4抗体[EPR15473]