重组Anti-Syntaxin 3抗体[EPR8543] (ab133750)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8543] to Syntaxin 3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Syntaxin 3抗体[EPR8543]
参阅全部 Syntaxin 3 一抗 -
描述
兔单克隆抗体[EPR8543] to Syntaxin 3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, Caco-2, , 293T, BxPC-3, Raw 264.7, PC-12, Rat lung, and NIH/3T3 lysates. IHC-P: human kidney, mouse kidney, and rat kidney tissues. ICC/IF: PC-12 and HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.34 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8543 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133750于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Potentially involved in docking of synaptic vesicles at presynaptic active zones. -
序列相似性
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6809 Human
- Entrez Gene: 20908 Mouse
- Entrez Gene: 81802 Rat
- Omim: 600876 Human
- SwissProt: Q13277 Human
- SwissProt: Q64704 Mouse
- SwissProt: Q08849 Rat
- Unigene: 180711 Human
see all -
别名
- FLJ30906 antibody
- Stx3 antibody
- STX3_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Syntaxin 3 with Purified ab133750 at 1:50 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 4 : Rat lung lysates
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : Raw 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH3T3 cell lysate
Lane 7 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Syntaxin 3 with ab133750 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of HepG2 cells labelling Syntaxin 3 with ab133750 at 1/50.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab133750 被引用在 16 文献中.
- Yamada K et al. Extended-Synaptotagmin 1 Enhances Liver Cancer Progression Mediated by the Unconventional Secretion of Cytosolic Proteins. Molecules 28:N/A (2023). PubMed: 37241771
- Hessvik NP et al. siRNA screening reveals that SNAP29 contributes to exosome release. Cell Mol Life Sci 80:177 (2023). PubMed: 37285022
- Li Q et al. A Functional Relationship Between UNC45A and MYO5B Connects Two Rare Diseases With Shared Enteropathy. Cell Mol Gastroenterol Hepatol 14:295-310 (2022). PubMed: 35421597
- Hess MW et al. Advanced Microscopy for Liver and Gut Ultrastructural Pathology in Patients with MVID and PFIC Caused by MYO5B Mutations. J Clin Med 10:N/A (2021). PubMed: 33924896
- Kalam SN et al. Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells. Reproduction 160:533-546 (2020). PubMed: 32698156