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RabMAb

Anti-Synip抗体[EP302Y] (ab40674)

Submit a review Q&A (1)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
  • Western blot - Anti-Synip antibody [EP302Y] (ab40674)

Key features and details

  • Rabbit monoclonal [EP302Y] to Synip
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Synip抗体[EP302Y]
    参阅全部 Synip 一抗

  • 描述

    兔单克隆抗体[EP302Y] to Synip

  • 宿主

    Rabbit

  • 特异性

    This antibody is specific for Synip. It is predicted to detect the isoform 2 based an sequence analysis.

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: ICC/IF or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human skeletal lysate and human lung carcinoma tissue.

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab40674于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/5000 - 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/5000 - 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC/IF or IP.

靶标

  • 功能

    Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.

  • 序列相似性

    Contains 1 PDZ (DHR) domain.
    Contains 1 WW domain.

  • 翻译后修饰

    Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • FLJ16496 antibody
    • MGC149829 antibody
    • MGC50337 antibody
    • OTTHUMP00000082667 antibody
    • OTTHUMP00000227400 antibody
    • STX4-interacting protein antibody
    • STXB4_HUMAN antibody
    • Stxbp4 antibody
    • Synip antibody
    • Syntaxin 4 interacting protein antibody
    • Syntaxin 4-interacting protein antibody
    • Syntaxin binding protein 4 antibody
    • Syntaxin-binding protein 4 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)

    ab40674 at a 1:250 dilution staining Synip in human lung carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Synip antibody [EP302Y] (ab40674)
    Western blot - Anti-Synip antibody [EP302Y] (ab40674)
    Anti-Synip antibody [EP302Y] (ab40674) at 1/5000 dilution + human skeletal lysate

    Predicted band size: 61 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab40674？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab40674 被引用在 3 文献中.

  • Otaka Y  et al. STXBP4 Drives Tumor Growth and Is Associated with Poor Prognosis through PDGF Receptor Signaling in Lung Squamous Cell Carcinoma. Clin Cancer Res 23:3442-3452 (2017). IHC . PubMed: 28087642
  • Taniai E  et al. Crosstalk between PTEN/Akt2 and TGFbeta signaling involving EGF receptor down-regulation during the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model. Cancer Sci 100:813-20 (2009). IHC-P ; Rat . PubMed: 19309364
  • Okada S  et al. Synip phosphorylation is required for insulin-stimulated Glut4 translocation. Biochem Biophys Res Commun 356:102-6 (2007). PubMed: 17336927

客户评价及客户问答

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Question

Hello, we are considering to purchase your TRAIL human ELISA Kit(Cat#: ab40674).
I want to detect the partial N-terminus(a.a114-281) of TRAIL by using the ELISA Kit.
But I wonder whether the Kit can detect the partial amino acid of TRAIL or not.
I have concerned about the TRAIL ELISA kit as it may have low specificity to detect parital TRAIL.
It's going to be helpful me with explanation if you don't have any above data I've mentioned.
Please reply to email asap since we are in hurry to carry out the experiment.
Thank you for your help.

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Answer

Thank you for contacting us.

The immunogen sequence of anti TRAIL antibody used in this kit corresponds to 114-281 of human TRAIL. It is more likely that it will detect your partial N-terminus (aa114-281) protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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