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Neuroscience Neurotransmission Intracellular Signaling Adapters

Anti-SynGAP抗体(ab3344)

  • Datasheet
Reviews (1) Submit a question References (5)

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Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)
  • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
  • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

Key features and details

  • Rabbit polyclonal to SynGAP
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SynGAP抗体
    参阅全部 SynGAP 一抗
  • 描述

    兔多克隆抗体to SynGAP
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Recombinant fragment within Rat SynGAP aa 900-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9QUH6-2

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Adapters
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3344于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 2 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.
说明
ICC/IF
Use a concentration of 2 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
  • 疾病相关

    Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
  • 序列相似性

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
  • 翻译后修饰

    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
  • Target information above from: UniProt accession Q96PV0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8831 Human
    • Entrez Gene: 192117 Rat
    • Omim: 603384 Human
    • SwissProt: Q96PV0 Human
    • SwissProt: Q9QUH6 Rat
    • Unigene: 586264 Human
    • Unigene: 9908 Rat
    • 别名

      • DKFZp761G1421 antibody
      • KIAA1938 antibody
      • MRD5 antibody
      • Neuronal RasGAP antibody
      • OTTHUMP00000064825 antibody
      • p135 SynGAP antibody
      • Ras GTPase activating protein SynGAP antibody
      • Ras GTPase-activating protein SynGAP antibody
      • RASA 1 antibody
      • RASA 5 antibody
      • RASA1 antibody
      • RASA5 antibody
      • SYGP1_HUMAN antibody
      • Synaptic Ras GAP 1 antibody
      • Synaptic Ras GTPase activating protein 1 antibody
      • Synaptic Ras GTPase activating protein 1 homolog antibody
      • Synaptic Ras GTPase activating protein 135kDa antibody
      • Synaptic Ras GTPase activating protein antibody
      • Synaptic Ras GTPase-activating protein 1 antibody
      • Synaptic Ras-GAP 1 antibody
      • SYNGAP 1 antibody
      • SYNGAP1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized PC-12 cells staining synGAP with ab3344 at 2 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody Alexa Fluor® 488 conjugate at 1:2000. Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue), and F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control is using no primary antibody control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)

      Immunohistochemical anysis of paraffin-embedded human hear tissue staining synGAP with ab3344 at 1/100 dilution counterstained with hematoxylin and detected using  HRP-conjugated secondary antibody. Negative control (left) has no primary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab3344?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3344 被引用在 5 文献中.

    • Dabrowska-Bouta B  et al. Early and Delayed Impact of Nanosilver on the Glutamatergic NMDA Receptor Complex in Immature Rat Brain. Int J Mol Sci 22:N/A (2021). PubMed: 33802775
    • McClatchy DB  et al. Quantitative analysis of global protein stability rates in tissues. Sci Rep 10:15983 (2020). PubMed: 32994440
    • Su P  et al. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal 12:N/A (2019). PubMed: 31387938
    • Bayés A  et al. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS One 7:e46683 (2012). PubMed: 23071613
    • Yang Y  et al. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience : (2011). WB, Electron Microscopy ; Rat . PubMed: 21736925

    客户评价及客户问答

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    Western blot abreview for Anti-SynGAP antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (Various ovarian cancer cell lines)
    Specification
    Various ovarian cancer cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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