Anti-SynGAP抗体(ab3344)
Key features and details
- Rabbit polyclonal to SynGAP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SynGAP抗体
参阅全部 SynGAP 一抗 -
描述
兔多克隆抗体to SynGAP -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment within Rat SynGAP aa 900-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9QUH6-2 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
|
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| IHC-P |
Use a concentration of 2 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 2 µg/ml. |
|
IHC-P
Use a concentration of 2 µg/ml. |
靶标
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功能
Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits. -
疾病相关
Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients. -
序列相似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain. -
翻译后修饰
Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. - Information by UniProt
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数据库链接
- Entrez Gene: 8831 Human
- Entrez Gene: 192117 Rat
- Omim: 603384 Human
- SwissProt: Q96PV0 Human
- SwissProt: Q9QUH6 Rat
- Unigene: 586264 Human
- Unigene: 9908 Rat
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别名
- DKFZp761G1421 antibody
- KIAA1938 antibody
- MRD5 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized PC-12 cells staining synGAP with ab3344 at 2 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody Alexa Fluor® 488 conjugate at 1:2000. Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue), and F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control is using no primary antibody control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)Immunohistochemical anysis of paraffin-embedded human hear tissue staining synGAP with ab3344 at 1/100 dilution counterstained with hematoxylin and detected using HRP-conjugated secondary antibody. Negative control (left) has no primary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.
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Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.
数据表及文件
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Datasheet download
文献 (5)
ab3344 被引用在 5 文献中.
- Dabrowska-Bouta B et al. Early and Delayed Impact of Nanosilver on the Glutamatergic NMDA Receptor Complex in Immature Rat Brain. Int J Mol Sci 22:N/A (2021). PubMed: 33802775
- McClatchy DB et al. Quantitative analysis of global protein stability rates in tissues. Sci Rep 10:15983 (2020). PubMed: 32994440
- Su P et al. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal 12:N/A (2019). PubMed: 31387938
- Bayés A et al. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS One 7:e46683 (2012). PubMed: 23071613
- Yang Y et al. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience : (2011). WB, Electron Microscopy ; Rat . PubMed: 21736925
