重组Anti-Syndecan-1抗体[SP152] (ab130405)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP152] to Syndecan-1
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Syndecan-1抗体[SP152]
参阅全部 Syndecan-1 一抗 -
描述
兔单克隆抗体[SP152] to Syndecan-1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Syndecan-1 aa 1-250. The exact sequence is proprietary.
Database link: P18827 -
阳性对照
- IHC-P: Human tonsil, Human skin, and Human hepatocellular carcinoma tissue; ICC: A431 cells. Flow Cyt: U266B1 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP152 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130405于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
Flow Cyt |
1/1000.
|
|
IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
1/1000. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
序列相似性
Belongs to the syndecan proteoglycan family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6382 Human
- Omim: 186355 Human
- SwissProt: P18827 Human
- Unigene: 224607 Human
- Unigene: 665958 Human
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别名
- CD_antigen antibody
- CD138 antibody
- CD138 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Syndecan-1 with purified ab130405 at 1:50 (5.36 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow Cytometry analysis of U266B1 (Human multiple myeloma B lymphocyte) cells labeling Syndecan-1 with purified ab130405 at 1/1000 dilution (1 µg/mL) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Gated on viable cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab130405, at a dilution of 1/100, staining Syndecan in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab130405 被引用在 2 文献中.
- Huang X et al. Heparanase Promotes Syndecan-1 Expression to Mediate Fibrillar Collagen and Mammographic Density in Human Breast Tissue Cultured ex vivo. Front Cell Dev Biol 8:599 (2020). PubMed: 32760722
- Luo B et al. Engineering of a-PD-1 antibody-expressing long-lived plasma cells by CRISPR/Cas9-mediated targeted gene integration. Cell Death Dis 11:973 (2020). PubMed: 33184267