AB60199

Anti-Syndecan-1抗体

Anti-Syndecan-1 antibody

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(9 Publications)

Rabbit Polyclonal Syndecan-1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 9 publications.

查看别名

CD138, SDC, SDC1, Syndecan-1, SYND1

2 Images

Unknown

Western blot - Anti-Syndecan-1 antibody (AB60199)

The observed band size is larger than predicted due to haparan sulfate and chondroitin sulfate modifications.

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Western blot - Anti-Syndecan-1 antibody (ab60199) at 4 µg/mL

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Mouse tissue lysate

Predicted band size: 32 kDa

Observed band size: 100 kDa

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PubMed

Western blot - Anti-Syndecan-1 antibody (AB60199)

Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.

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Western blot - Anti-Syndecan-1 antibody (ab60199) at 1/2000 dilution

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lysate prepared from ScN2a cells.

Secondary

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peroxidase-conjugated secondary

Predicted band size: 32 kDa

true

Image from Taylor DR et al, PLoS Pathog. 2009 Nov;5(11):e1000666. Epub 2009 Nov 20, Fig 6.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse

应用

applications

免疫原

Synthetic Peptide within Human SDC1. The exact immunogen used to generate this antibody is proprietary information.

P18827

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "<p></p>" } } }

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产品详情

This product is manufactured by BioVision, an Abcam company and was previously called 3643R Syndecan-1 Antibody. 3643R-100 is the same size as the 100 µg size of ab60199.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.

Biological function summary

Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.

Pathways

Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.

Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity). Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed : 22660413). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity).

See full target information SDC1

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35008785

2021

Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Guang Yu,Vimala Bondada,Hina Iqbal,Kate L Moore,John C Gensel,Subbarao Bondada,James W Geddes

Cancer management and research 13:2885-2896 PubMed33833571

2021

circCEP128 Knockdown Suppresses Bladder Cancer Progression via Regulating microRNA-515-5p/SDC1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Cao,Chan Zhang,Xiangyong Tian,Gaopeng Jing,Xiaolin Zhou,Tianzhong Yan

Cell biology international 44:894-904 PubMed31868265

2020

Phosphorylation of Dab2 is involved in inhibited VEGF-VEGFR-2 signaling induced by downregulation of syndecan-1 in glomerular endothelial cell.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Jing,Wu Jia-Jun,Yuan Wei-Jie

The Journal of clinical investigation 125:2877-90 PubMed26098211

2015

Interferon-induced mechanosensing defects impede apoptotic cell clearance in lupus.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Li,Yang-Xin Fu,Qi Wu,Yong Zhou,David K Crossman,PingAr Yang,Jun Li,Bao Luo,Laurence M Morel,Janusz H Kabarowski,Hideo Yagita,Carl F Ware,Hui-Chen Hsu,John D Mountz

PloS one 9:e89824 PubMed24587062

2014

Syndecan-1 regulates vascular smooth muscle cell phenotype.

Applications

Unspecified application

Species

Mouse

Somali Chaterji,Christoffer H Lam,Derek S Ho,Daniel C Proske,Aaron B Baker

Molecular human reproduction 20:567-78 PubMed24478463

2014

Syndecan-1 knockdown in endometrial epithelial cells alters their apoptotic protein profile and enhances the inducibility of apoptosis.

Applications

Unspecified application

Species

Human

S J Boeddeker,D M Baston-Buest,O Altergot-Ahmad,J S Kruessel,A P Hess

Investigative ophthalmology & visual science 54:6373-81 PubMed23989188

2013

An investigative peptide-acyclovir combination to control herpes simplex virus type 1 ocular infection.

Applications

Unspecified application

Species

Unspecified reactive species

Paul J Park,Thessicar E Antoine,Asim V Farooq,Tibor Valyi-Nagy,Deepak Shukla

Development (Cambridge, England) 138:4523-33 PubMed21903674

2011

Alpha sarcoglycan is required for FGF-dependent myogenic progenitor cell proliferation in vitro and in vivo.

Applications

Species

Mouse

Marco Cassano,Arianna Dellavalle,Francesco Saverio Tedesco,Mattia Quattrocelli,Stefania Crippa,Flavio Ronzoni,Agnese Salvade,Emanuele Berardi,Yvan Torrente,Giulio Cossu,Maurilio Sampaolesi

PLoS pathogens 5:e1000666 PubMed19936054

2009

Glypican-1 mediates both prion protein lipid raft association and disease isoform formation.

Applications

Species

Mouse

David R Taylor,Isobel J Whitehouse,Nigel M Hooper

View all publications

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Anti-Syndecan-1抗体

Anti-Syndecan-1 antibody

Western blot - Anti-Syndecan-1 antibody (AB60199)

Western blot - Anti-Syndecan-1 antibody (AB60199)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

替代产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

搭配使用产品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (9)

Abcam Product Promise