Anti-Synaptopodin抗体(ab224491)
Key features and details
- Rabbit polyclonal to Synaptopodin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Synaptopodin抗体
参阅全部 Synaptopodin 一抗 -
描述
兔多克隆抗体to Synaptopodin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Synaptopodin aa 450-550.
Database link: Q8N3V7 -
阳性对照
- WB: Human skeletal muscle tissue lysate. IHC-P: Human kidney, cerebral cortex and skeletal muscle tissues. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224491于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa.
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| IHC-P | (1) |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa. |
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity. -
组织特异性
Expressed in cerebral cortex. -
序列相似性
Belongs to the synaptopodin family. -
翻译后修饰
O-glycosylated.
Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871. -
细胞定位
Cytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses. - Information by UniProt
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数据库链接
- Entrez Gene: 11346 Human
- Omim: 608155 Human
- SwissProt: Q8N3V7 Human
- Unigene: 435228 Human
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别名
- KIAA1029 antibody
- Synaptopodin antibody
- SYNPO antibody
- SYNPO_HUMAN antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (ab224491)
Paraffin-embedded human kidney tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (ab224491)Paraffin-embedded human cerebral cortex tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (ab224491)Paraffin-embedded human skeletal muscle tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody (ab224491)Negative control: Paraffin-embedded human tonsil tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Western blot - Anti-Synaptopodin antibody (ab224491)Anti-Synaptopodin antibody (ab224491) at 1/100 dilution + Human skeletal muscle tissue lysate
Developed using the ECL technique.
Predicted band size: 99 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Synaptopodin antibody (ab224491)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Synaptopodin (green) using ab224491 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab224491 被引用在 13 文献中.
- Murphy C et al. Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation. ALTEX 40:141-159 (2023). PubMed: 35791294
- Zhu Y et al. Polynucleotide phosphorylase protects against renal tubular injury via blocking mt-dsRNA-PKR-eIF2α axis. Nat Commun 14:1223 (2023). PubMed: 36869030
- Zeng Y et al. Effects and mechanism of Rictor interference in podocyte injury induced by high glucose. Exp Ther Med 26:473 (2023). PubMed: 37753299
- Bejoy J et al. Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease. Stem Cell Res Ther 13:355 (2022). PubMed: 35883199
- Shen Q et al. Integrated genomic, transcriptomic and metabolomic analysis reveals MDH2 mutation-induced metabolic disorder in recurrent focal segmental glomerulosclerosis. Front Immunol 13:962986 (2022). PubMed: 36159820