Anti-Synaptophysin抗体[SY38] (ab8049)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)](https://www.abcam.cn/ps/products/8/ab8049/Images/ab8049-290861-anti-synaptophysin-antibody-sy38-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)")
Key features and details
- Mouse monoclonal [SY38] to Synaptophysin
- Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Hamster, Cow, Human
- Isotype: IgG1
概述
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产品名称
Anti-Synaptophysin抗体[SY38]
参阅全部 Synaptophysin 一抗 -
描述
小鼠单克隆抗体[SY38] to Synaptophysin -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Hamster, Cow, Human -
免疫原
Full length native protein (purified) corresponding to Bovine Synaptophysin. Synaptophysin from presynaptic vesicles, prepared from bovine brain.
Database link: P20488 -
阳性对照
- Adrenal pheochromocytoma and pancreatic islet cell neoplasm
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. -
存储溶液
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SY38 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt | (1) |
1/20.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| IHC-P | (6) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
1/20. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
组织特异性
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
疾病相关
Mental retardation, X-linked, SYP-related -
序列相似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
结构域
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 280937 Cow
- Entrez Gene: 6855 Human
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- Omim: 313475 Human
- SwissProt: P20488 Cow
- SwissProt: P08247 Human
- SwissProt: Q62277 Mouse
see all -
别名
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs
ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
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![Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)](https://www.abcam.cn/ps/products/8/ab8049/Images/ab8049-13492-anti-synaptophysin-antibody-sy38-western-blot.jpg)
Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)](https://www.abcam.cn/ps/products/8/ab8049/Images/ab8049-256710-anti-synaptophysin-antibody-sy38-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs.ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)](https://www.abcam.cn/ps/products/8/ab8049/Images/ab8049-13493-anti-synaptophysin-antibody-sy38-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine. The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,
secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.
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![Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)](https://www.abcam.cn/ps/products/8/ab8049/Images/ab8049-13491-anti-synaptophysin-antibody-sy38-flow-cytometry.jpg)
Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (228)
ab8049 被引用在 228 文献中.
- Sun X et al. Bone marrow mesenchymal stem cells and exercise restore motor function following spinal cord injury by activating PI3K/AKT/mTOR pathway. Neural Regen Res 18:1067-1075 (2023). PubMed: 36254995
- Liu Q et al. Electric field stimulation boosts neuronal differentiation of neural stem cells for spinal cord injury treatment via PI3K/Akt/GSK-3β/β-catenin activation. Cell Biosci 13:4 (2023). PubMed: 36624495
- Tang F et al. Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels. Neurochem Res 48:791-803 (2023). PubMed: 36335177
- Lang HL et al. Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural Regen Res 18:609-617 (2023). PubMed: 36018185
- Kuan WL et al. Selective neurodegeneration generated by intravenous α-synuclein pre-formed fibril administration is not associated with endogenous α-synuclein levels in the rat brain. Brain Pathol 33:e13128 (2023). PubMed: 36321260