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Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs

Anti-Synaptophysin抗体[SY38] (ab8049)

  • Datasheet
  • SDS
Reviews (15)Q&A (11)References (228)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
  • Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
  • Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)

Key features and details

  • Mouse monoclonal [SY38] to Synaptophysin
  • Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Hamster, Cow, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Synaptophysin抗体[SY38]
    参阅全部 Synaptophysin 一抗
  • 描述

    小鼠单克隆抗体[SY38] to Synaptophysin
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Hamster, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Bovine Synaptophysin. Synaptophysin from presynaptic vesicles, prepared from bovine brain.
    Database link: P20488

  • 阳性对照

    • Adrenal pheochromocytoma and pancreatic islet cell neoplasm
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C.
  • 存储溶液

    pH: 7.3
    Preservative: 0.09% Sodium azide
    Constituents: 0.5% BSA, PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    SY38
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Synaptophysin protein (ab114166)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab8049于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
ICC/IF (2)
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
Flow Cyt (1)
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (6)
Use at an assay dependent concentration.
说明
WB
1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • 组织特异性

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • 疾病相关

    Mental retardation, X-linked, SYP-related
  • 序列相似性

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • 结构域

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • 翻译后修饰

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Target information above from: UniProt accession P08247 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 280937 Cow
    • Entrez Gene: 6855 Human
    • Entrez Gene: 20977 Mouse
    • Entrez Gene: 24804 Rat
    • Omim: 313475 Human
    • SwissProt: P20488 Cow
    • SwissProt: P08247 Human
    • SwissProt: Q62277 Mouse
    • SwissProt: P07825 Rat
    • Unigene: 632804 Human
    • Unigene: 223674 Mouse
    • Unigene: 11067 Rat
    see all
  • 别名

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs

    ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)
    Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)
    All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs.

    ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)

    The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine.  The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,  
    secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

    These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.

  • Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)
    Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)
    Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (228)

发表研究结果有使用 ab8049?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab8049 被引用在 228 文献中.

  • Sun X  et al. Bone marrow mesenchymal stem cells and exercise restore motor function following spinal cord injury by activating PI3K/AKT/mTOR pathway. Neural Regen Res 18:1067-1075 (2023). PubMed: 36254995
  • Liu Q  et al. Electric field stimulation boosts neuronal differentiation of neural stem cells for spinal cord injury treatment via PI3K/Akt/GSK-3β/β-catenin activation. Cell Biosci 13:4 (2023). PubMed: 36624495
  • Tang F  et al. Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels. Neurochem Res 48:791-803 (2023). PubMed: 36335177
  • Lang HL  et al. Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural Regen Res 18:609-617 (2023). PubMed: 36018185
  • Kuan WL  et al. Selective neurodegeneration generated by intravenous α-synuclein pre-formed fibril administration is not associated with endogenous α-synuclein levels in the rat brain. Brain Pathol 33:e13128 (2023). PubMed: 36321260
View all Publications for this product

客户评价及客户问答

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1-10 of 26 Abreviews or Q&A

IHC - Wholemount abreview for Anti-Synaptophysin antibody [SY38]

Below Average
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 Mouse Embryo)
Specification
E12.5 Mouse Embryo
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alain Chedotal

Verified customer

提交于 Jan 31 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 10 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 10 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 May 16 2016

IHC - Wholemount abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Skeletal muscle)
Specification
Skeletal muscle
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 08 2016

Immunocytochemistry abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (cortical neurons primary culture)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
cortical neurons primary culture
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 01 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [SY38]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Dec 22 2015

Flow Cytometry abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 15 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (Differentiated hNSCs)
Specification
Differentiated hNSCs
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 16 2015

Immunohistochemistry (Frozen sections) abreview for Anti-Synaptophysin antibody [SY38]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Mouse Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 20 2015

1-10 of 26 Abreviews or Q&A

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