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重组RabMAb

重组Anti-Synaptophysin抗体[EPR1097-2] (ab178412)

Submit a review Submit a question References (2)
Western blot - Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
  • Western blot - Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
  • Anti-Synaptophysin antibody [EPR1097-2] (ab178412)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1097-2] to Synaptophysin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Synaptophysin抗体[EPR1097-2]
    参阅全部 Synaptophysin 一抗

  • 描述

    兔单克隆抗体[EPR1097-2] to Synaptophysin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Rat brain, PC12, Mouse brain and Human brain lysates; PC12 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab178412于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
说明
WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.

  • 组织特异性

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

  • 疾病相关

    Mental retardation, X-linked, SYP-related

  • 序列相似性

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.

  • 结构域

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

  • 翻译后修饰

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

  • 细胞定位

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

图片

  • Western blot - Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
    Western blot - Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
    Anti-Synaptophysin antibody [EPR1097-2] (ab178412) at 1/1000 dilution (Purified) + Rat brain lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
    Western blot - Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
    All lanes : Anti-Synaptophysin antibody [EPR1097-2] (ab178412) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?

  • Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
    Anti-Synaptophysin antibody [EPR1097-2] (ab178412)

文献 (2)

发表研究结果有使用 ab178412？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab178412 被引用在 2 文献中.

  • Lin M  et al. Establishment of gastrointestinal assembloids to study the interplay between epithelial crypts and their mesenchymal niche. Nat Commun 14:3025 (2023). PubMed: 37230989
  • Udomruk S  et al. Sesamin Promotes Neurite Outgrowth under Insufficient Nerve Growth Factor Condition in PC12 Cells through ERK1/2 Pathway and SIRT1 Modulation. Evid Based Complement Alternat Med 2020:9145458 (2020). PubMed: 32308720

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