Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
参阅全部 Synaptophysin 一抗
特异性This antibody reacts with a hydrophilic peptide sequence of synaptophysin.
经测试应用适用于: ICC/IFmore details
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
- ICC/IF: PC12 Cells
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
纯度Immunogen affinity purified
The Abpromise guarantee
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml.
功能Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
组织特异性Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
疾病相关Mental retardation, X-linked, SYP-related
序列相似性Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain.
结构域The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
翻译后修饰Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
- Information by UniProt
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
ICC/IF image of ab7837 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7837, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab7837 被引用在 11 文献中.
- Kourgiantaki A et al. Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury. NPJ Regen Med 5:12 (2020). PubMed: 32566251
- Zeineldin M et al. MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Nat Commun 11:913 (2020). PubMed: 32060267
- Gan Q et al. Effects of Amylin Against Amyloid-ß-Induced Tauopathy and Synapse Loss in Primary Neurons. J Alzheimers Dis 70:1025-1040 (2019). PubMed: 31306122
- Pathak NM et al. Novel dual incretin agonist peptide with antidiabetic and neuroprotective potential. Biochem Pharmacol 155:264-274 (2018). PubMed: 30028989
- Denver P et al. Inflammation, insulin signaling and cognitive function in aged APP/PS1 mice. Brain Behav Immun 70:423-434 (2018). PubMed: 29604345