Anti-Synaptophysin抗体(ab32594)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Synaptophysin抗体
参阅全部 Synaptophysin 一抗 -
描述
兔多克隆抗体to Synaptophysin -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat
预测可用于: Human
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免疫原
Synthetic peptide:
GPGGYGPQDSYGPQGGYQPD
, corresponding to amino acids 253-272 of Rat Synaptophysin -
阳性对照
- WB: Mouse and rat brain tissue lysates. ICC/IF: rat hippocampal neurons
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa).
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
组织特异性
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
疾病相关
Mental retardation, X-linked, SYP-related -
序列相似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
结构域
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 6855 Human
- Entrez Gene: 24804 Rat
- Omim: 313475 Human
- SwissProt: P08247 Human
- SwissProt: P07825 Rat
- Unigene: 632804 Human
- Unigene: 11067 Rat
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别名
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
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Western blot - Anti-Synaptophysin antibody (ab32594)All lanes : Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDaWestern blot analysis was performed on tissue extracts (30 µg lysate). The blot was probed with ab32594 (1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A 34 kDa band corresponding to Synaptophysin was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be Synaptophysin negative.
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Western blot - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous AbreviewAnti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg
Secondary
AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG
at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 33,35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
-
Western blot - Anti-Synaptophysin antibody (ab32594)Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain
Predicted band size: 34 kDa
Observed band size: 33.8 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (60)
ab32594 被引用在 60 文献中.
- Guo Y et al. Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats. Neural Regen Res 18:1090-1098 (2023). PubMed: 36254998
- Li Y et al. Multi-omics analysis of a drug-induced model of bipolar disorder in zebrafish. iScience 26:106744 (2023). PubMed: 37207274
- Tyrtyshnaia A et al. Synaptamide Ameliorates Hippocampal Neurodegeneration and Glial Activation in Mice with Traumatic Brain Injury. Int J Mol Sci 24:N/A (2023). PubMed: 37373162
- Reifschneider A et al. Loss of TREM2 rescues hyperactivation of microglia, but not lysosomal deficits and neurotoxicity in models of progranulin deficiency. EMBO J 41:e109108 (2022). PubMed: 35019161
- Kútna V et al. Cerebellar demyelination and neurodegeneration associated with mTORC1 hyperactivity may contribute to the developmental onset of autism-like neurobehavioral phenotype in a rat model. Autism Res 15:791-805 (2022). PubMed: 35178882