Anti-Synapsin I抗体- Synaptic Marker (ab64581)
Key features and details
- Rabbit polyclonal to Synapsin I - Synaptic Marker
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Goat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Synapsin I抗体- Synaptic Marker
参阅全部 Synapsin I 一抗 -
描述
兔多克隆抗体to Synapsin I - Synaptic Marker -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab64581 are tested in WB. Previous batches were additionally validated in IP. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab254349.
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经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Goat
预测可用于: Sheep, Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64581于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa).
Abcam recommends using 3-5% milk as the blocking agent. |
| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa). Abcam recommends using 3-5% milk as the blocking agent. |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
疾病相关
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
序列相似性
Belongs to the synapsin family. -
翻译后修饰
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
- SwissProt: P09951 Rat
- Unigene: 225936 Human
see all -
别名
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
图片
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Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image is courtesy of an anonymous AbreviewAnti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution + Rat whole brain tissue lysate at 15 µg
Secondary
Donkey anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocked with 5% milk for 1 hour at 25°C.
Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.
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Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)All lanes : Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?
The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data). -
Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (110)
ab64581 被引用在 110 文献中.
- Shi CJ et al. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Acta Pharmacol Sin 44:92-104 (2023). PubMed: 35794374
- Ge J et al. MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice. JCI Insight 8:N/A (2023). PubMed: 37192007
- Liao Z et al. Involvement of IL-1β-Mediated Necroptosis in Neurodevelopment Impairment after Neonatal Sepsis in Rats. Int J Mol Sci 24:N/A (2023). PubMed: 37834141
- Fu Y et al. Hippocampal BAIAP2 prevents chronic mild stress-induced depression-like behaviors in mice. Front Psychiatry 14:1192379 (2023). PubMed: 37234209
- Mukhamedyarov MA et al. Early Alterations in Structural and Functional Properties in the Neuromuscular Junctions of Mutant FUS Mice. Int J Mol Sci 24:N/A (2023). PubMed: 37240370