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重组RabMAb

重组Anti-Synapsin I抗体[EPR23531-50] - BSA and Azide free (ab274430)

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Multiplex immunohistochemistry - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Flow Cytometry (Intracellular) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
  • Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23531-50] to Synapsin I - BSA and Azide free
  • Suitable for: mIHC, IHC-Fr, IHC-P, Flow Cyt (Intra), ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-Synapsin I抗体[EPR23531-50] - BSA and Azide free
    参阅全部 Synapsin I 一抗
  • 描述

    兔单克隆抗体[EPR23531-50] to Synapsin I - BSA and Azide free
  • 宿主

    Rabbit
  • 经测试应用

    适用于: mIHC, IHC-Fr, IHC-P, Flow Cyt (Intra), ICC/IF, WB, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse brain tissue lysate; Rat brain and spinal cord tissue lysates; Human brain tissue lysate. IHC-P: Human hippocampus tissue; Mouse hippocampus and pancreas tissue; Rat retina tissue. IHC-Fr: Mouse cerebrum and retina tissue; rat cerebrum and retina tissue. ICC/IF: Mouse primary neuron cells; Rat primary neuron cells. Flow Cyt (intra): Mouse primary brain cells. IP: Mouse brain tissue lysate; Rat brain tissue lysate. mIHC: Human retina tissue.
  • 常规说明

    ab274430 is the carrier-free version of ab254349.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    pH: 7.2
    Constituent: PBS
  • 无载体

    是
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR23531-50
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Processes
    • Cell Biology
    • Other Antibodies
    • mIHC validated

相关产品

  • Alternative Versions

    • Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (ab254349)
    • Alexa Fluor® 647 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (ab305212)
    • Alexa Fluor® 488 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (ab307130)
    • Alexa Fluor® 555 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50] (ab309971)
    • Alexa Fluor® 594 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50] (ab309972)
    • PE Anti-Synapsin I antibody [EPR23531-50] – Synaptic Marker (ab317882)
    • APC Anti-Synapsin I antibody [EPR23531-50] – Synaptic Marker (ab317918)
    • Alexa Fluor® 750 Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (ab321505)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Related Products

    • Anti-MAP2 antibody [HM-2] (ab11267)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab274430于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
mIHC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70, 75 kDa (predicted molecular weight: 74 kDa).
IP
Use at an assay dependent concentration.
说明
mIHC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70, 75 kDa (predicted molecular weight: 74 kDa).
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • 疾病相关

    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • 序列相似性

    Belongs to the synapsin family.
  • 翻译后修饰

    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位

    Cell junction > synapse. Golgi apparatus.
  • Target information above from: UniProt accession P17600 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6853 Human
    • Entrez Gene: 20964 Mouse
    • Entrez Gene: 24949 Rat
    • Omim: 313440 Human
    • SwissProt: P17600 Human
    • SwissProt: O88935 Mouse
    • SwissProt: P09951 Rat
    • Unigene: 225936 Human
    • Unigene: 439844 Mouse
    • Unigene: 9923 Rat
    see all
  • 别名

    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

图片

  • Multiplex immunohistochemistry - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Multiplex immunohistochemistry - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human retina tissue labeling RPE65, Rhodopsin and Synapsin I with ab231782 at 1/8000 dilution, ab232934 at 1/8000 dilution and ab274430 at 1/1500 dilution followed by a ready to use Opal Polymer HRP Ms + Rb secondary antibody. Nuclear counter stain used was DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    Panel A: merged staining of anti-Synapsin I (magenta; Opal™690), anti-RPE65 (green; Opal™520) and anti-Rhodopsin (red; Opal™570) on human retina.
    Panel B: anti-RPE65 stained on pigmented layer.
    Panel C: anti-Rhodopsin stained on rod photoreceptor cells.
    Panel D: anti-Synapsin I stained on inner plexiform layer.

    The section was incubated in three rounds of staining: in the order of ab274430, ab231782, and ab232934 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    The section was incubated in three rounds of staining: in the order of ab312840, ab16669, and ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Synapsin-1 with ab254349 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab254349 at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling Synapsin-1 with ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human hippocampus (PMID: 22900032). The section was incubated with ab254349 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling Synapsin-1 with ab254349 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse retina is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    All lanes : Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (ab254349) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat spinal cord tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 74 kDa



    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Mouse lung (PMID: 9539796).

    Exposure times: Lanes 1-3: 5.5 seconds; Lane 4: 3 minutes.

  • Flow Cytometry (Intracellular) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Flow Cytometry (Intracellular) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse primary brain cells cells labelling Synapsin-1 with ab254349 at 1/50 dilution (1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Synapsin-1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab254349 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254349 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab254349 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254349 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

     

  • Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (ab254349) at 1/1000 dilution + Human brain tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 74 kDa
    Observed band size: 70,75 kDa why is the actual band size different from the predicted?



    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 15 seconds.

  • Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    All lanes : Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (ab254349) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spinal cord tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 74 kDa
    Observed band size: 70,75 kDa why is the actual band size different from the predicted?



    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 15 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Synapsin-1 with ab254349 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Synapsin-1 with ab254349 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat retina tissue labeling Synapsin-1 with ab254349 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat retina is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling Synapsin-1 with ab254349 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on mouse lung (PMID: 9539796) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling Synapsin-1 with ab254349 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on rat lung (PMID: 9539796) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Synapsin-1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab254349 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254349 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Rat brain tissue lysate 10 ug

    Lane 2: ab254349 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254349 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neuron cells labelling Synapsin-1 with ab254349 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab254349 at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Synapsin-1 with ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse hippocampus (PMID: 22900032). The section was incubated with ab254349 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Synapsin-1 with ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse pancreatic islets (PMID: 22334712). The section was incubated with ab254349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

    This data was developed using ab254349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling Synapsin-1 with ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat retina (PMID: 2124189). The section was incubated with ab254349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
    Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

文献 (1)

发表研究结果有使用 ab274430?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab274430 被引用在 1 文献中.

  • Kim HJ  et al. Brain-Derived Neurotrophic Factor Secreting Human Mesenchymal Stem Cells Improve Outcomes in Rett Syndrome Mouse Models. Front Neurosci 15:725398 (2021). PubMed: 34690674

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