Anti-Synapsin I抗体(ab18814)
Key features and details
- Rabbit polyclonal to Synapsin I
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Synapsin I抗体
参阅全部 Synapsin I 一抗 -
描述
兔多克隆抗体to Synapsin I -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Synapsin I. Corresponds to residues surrounding amino acid 12 of Synapsin I.
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 6008 Synapsin Antibody. 6008-100 is the same size as the 100 µg size of ab18814.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18814于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 74 kDa).
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 74 kDa). |
靶标
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功能
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
疾病相关
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
序列相似性
Belongs to the synapsin family. -
翻译后修饰
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 6853 Human
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: P09951 Rat
- Unigene: 225936 Human
- Unigene: 9923 Rat
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别名
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
图片
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Western blot - Anti-Synapsin I antibody (ab18814)All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution
Lane 1 : Human brain whole cell lysate - untreated
Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).
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Western blot - Anti-Synapsin I antibody (ab18814)All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution
Lane 1 : Rat brain whole cell lysate - untreated
Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab18814 被引用在 14 文献中.
- Shi H et al. Supplement of microbiota-accessible carbohydrates prevents neuroinflammation and cognitive decline by improving the gut microbiota-brain axis in diet-induced obese mice. J Neuroinflammation 17:77 (2020). PubMed: 32127019
- Shen Y et al. Ultrasound with microbubbles improves memory, ameliorates pathology and modulates hippocampal proteomic changes in a triple transgenic mouse model of Alzheimer's disease. Theranostics 10:11794-11819 (2020). PubMed: 33052247
- Xu P et al. Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke. Cell Death Dis 10:555 (2019). PubMed: 31324751
- Dzhashiashvili Y et al. The UPR-PERK pathway is not a promising therapeutic target for mutant SOD1-induced ALS. Neurobiol Dis 127:527-544 (2019). PubMed: 30923003
- Wheatley EG et al. Neuronal O-GlcNAcylation Improves Cognitive Function in the Aged Mouse Brain. Curr Biol 29:3359-3369.e4 (2019). PubMed: 31588002