Anti-SV40 VP2 + VP3抗体(ab53983)
Key features and details
- Rabbit polyclonal to SV40 VP2 + VP3
- Suitable for: WB
- Reacts with: Simian Virus 40
- Isotype: IgG
概述
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产品名称
Anti-SV40 VP2 + VP3抗体 -
描述
兔多克隆抗体to SV40 VP2 + VP3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Simian Virus 40 -
免疫原
Recombinant fragment corresponding to SV40 VP2 + VP3.
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常规说明
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: Whole serum, 50% Glycerol -
Concentration information loading... -
纯度
Whole antiserum -
Primary antibody说明
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3 -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53983于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 38,26 kDa.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 38,26 kDa. |
靶标
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相关性
There are three simian virus 40 (SV40) capsid proteins - VP1, VP2 and VP3. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid. -
数据库链接
- SwissProt: P03093 Simian Virus 40
图片
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Western blot - Anti-SV40 VP2 + VP3 antibody (ab53983)This image is courtesy of an anonymous AbreviewAll lanes : Anti-SV40 VP2 + VP3 antibody (ab53983) at 1/5000 dilution
Lane 1 : BK virus infected 293T cell lysate
Lane 2 : Mock 293T cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38,26 kDa
Observed band size: 27,38 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
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Western blot - Anti-SV40 VP2 + VP3 antibody (ab53983)SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab53983 被引用在 20 文献中.
- Spriggs CC et al. Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection. PLoS Pathog 18:e1010824 (2022). PubMed: 36067270
- Bagchi P et al. Lunapark-dependent formation of a virus-induced ER exit site contains multi-tubular ER junctions that promote viral ER-to-cytosol escape. Cell Rep 37:110077 (2021). PubMed: 34879280
- Spriggs CC et al. Golgi-associated BICD adaptors couple ER membrane penetration and disassembly of a viral cargo. J Cell Biol 219:N/A (2020). PubMed: 32259203
- Panou MM et al. Glibenclamide inhibits BK polyomavirus infection in kidney cells through CFTR blockade. Antiviral Res 178:104778 (2020). PubMed: 32229236
- Chen YJ et al. Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. J Cell Biol 219:N/A (2020). PubMed: 31895406