Anti-SV40 VP1抗体(ab53977)
Key features and details
- Rabbit polyclonal to SV40 VP1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Simian Virus 40
- Isotype: IgG
概述
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产品名称
Anti-SV40 VP1抗体 -
描述
兔多克隆抗体to SV40 VP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Simian Virus 40 -
免疫原
The details of the immunogen for this antibody are not available.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53977于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/10000. Predicted molecular weight: 40 kDa.
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| IP |
Use at an assay dependent dilution.
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| ICC/IF |
1/1000 - 1/5000.
|
| 说明 |
|---|
|
WB
1/10000. Predicted molecular weight: 40 kDa. |
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IP
Use at an assay dependent dilution. |
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ICC/IF
1/1000 - 1/5000. |
靶标
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相关性
VP1 is one of three simian virus 40 (SV40) capsid proteins. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid. -
数据库链接
- SwissProt: P03087 Simian Virus 40
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别名
- Capsid protein VP1 antibody
图片
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Western blot - Anti-SV40 VP1 antibody (ab53977)All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution
Lane 1 : BK virus infected 293TT whole cell lysate
Lane 2 : Mock infected 293TT whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
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Western blot - Anti-SV40 VP1 antibody (ab53977)SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3 -
Immunoprecipitation - Anti-SV40 VP1 antibody (ab53977)ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method. -
Immunocytochemistry/ Immunofluorescence - Anti-SV40 VP1 antibody (ab53977)ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.
实验方案
数据表及文件
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Datasheet download
文献 (16)
ab53977 被引用在 16 文献中.
- Spriggs CC et al. Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection. PLoS Pathog 18:e1010824 (2022). PubMed: 36067270
- Sikorski M et al. Non-permissive human conventional CD1c+ dendritic cells enable trans-infection of human primary renal tubular epithelial cells and protect BK polyomavirus from neutralization. PLoS Pathog 17:e1009042 (2021). PubMed: 33592065
- Bagchi P et al. Lunapark-dependent formation of a virus-induced ER exit site contains multi-tubular ER junctions that promote viral ER-to-cytosol escape. Cell Rep 37:110077 (2021). PubMed: 34879280
- Motamedi N et al. SV40 Polyomavirus Activates the Ras-MAPK Signaling Pathway for Vacuolization, Cell Death, and Virus Release. Viruses 12:N/A (2020). PubMed: 33028008
- Tsai K et al. Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication. PLoS Pathog 14:e1006919 (2018). PubMed: 29447282
