Anti-SV40 VP1 抗体

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SV40 VP1 antibody (AB53977)

ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.

• IP

Unknown

Immunoprecipitation - Anti-SV40 VP1 antibody (AB53977)

ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 μg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.

All lanes:

Immunoprecipitation - Anti-SV40 VP1 antibody (ab53977)

Predicted band size: 40 kDa

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• WB

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Western blot - Anti-SV40 VP1 antibody (AB53977)

SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3

Lane 1:

Western blot - Anti-SV40 VP1 antibody (ab53977)

Lane 1:

Western blot - Anti-SV40 VP2 + VP3 antibody (<a href='/products/primary-antibodies/sv40-vp2-vp3-antibody-ab53983'>ab53983</a>)

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• WB

AbReview46247****

Western blot - Anti-SV40 VP1 antibody (AB53977)

All lanes:

Western blot - Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution

Lane 1:

BK virus infected 293TT whole cell lysate at 15 µg

Lane 2:

Mock infected 293TT whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 40 kDa

Observed band size: 45 kDa

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Exposure time: 4min