Anti-Survivin抗体(ab469)
Key features and details
- Rabbit polyclonal to Survivin
- Suitable for: ICC/IF, WB, IHC-P, ELISA, Flow Cyt, IP, RIP
- Reacts with: Mouse, Rat, Cow, Cat, Dog, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Survivin抗体
参阅全部 Survivin 一抗 -
描述
兔多克隆抗体to Survivin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, ELISA, Flow Cyt, IP, RIPmore details -
种属反应性
与反应: Mouse, Rat, Cow, Cat, Dog, Human -
免疫原
Recombinant full length protein corresponding to Human Survivin .
Database link: O15392 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.876% Sodium chloride, 99% Tris glycine -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
1/250. See Abreview by William Moore; fix with formaldehyde.
|
| WB | (4) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution.
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| IHC-P | (3) |
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| ELISA |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | (1) |
Use at an assay dependent concentration.
Recommended to use at 5-7µg/ml. |
| RIP |
Use at an assay dependent concentration. PubMed: 19542185
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| 说明 |
|---|
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ICC/IF
1/250. See Abreview by William Moore; fix with formaldehyde. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution. |
|
IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. Recommended to use at 5-7µg/ml. |
|
RIP
Use at an assay dependent concentration. PubMed: 19542185 |
靶标
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功能
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. -
组织特异性
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. -
序列相似性
Belongs to the IAP family.
Contains 1 BIR repeat. -
发展阶段
Expression is cell cycle-dependent and peaks at mitosis. -
结构域
The BIR repeat is necessary and sufficient for HBXIP binding. -
翻译后修饰
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
细胞定位
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 493835 Cat
- Entrez Gene: 414925 Cow
- Entrez Gene: 442936 Dog
- Entrez Gene: 332 Human
- Entrez Gene: 11799 Mouse
- Entrez Gene: 64041 Rat
- Omim: 603352 Human
- SwissProt: Q6I6F4 Cat
see all -
别名
- API4 antibody
- Apoptosis inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
图片
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Western blot - Anti-Survivin antibody (ab469)All lanes : Anti-Survivin antibody (ab469) at 1 µg/ml
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 µg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.
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Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)This image is courtesy of an Abreview submitted by Dr Ben Davidsonab469 staining Survivin from Human Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)This image is courtesy of William MooreHeLa cells (ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK.
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Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.
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Western blot - Anti-Survivin antibody (ab469)Anti-Survivin antibody (ab469) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 16 kDa
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)This image is courtesy of an Abreview submitted by Mr. Rudolf Jung.Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma tissue stained for Survivin using ab469 at 1/500 dilution in immunohistochemical analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (134)
ab469 被引用在 134 文献中.
- Zuco V et al. Effectiveness of irinotecan plus trabectedin on a desmoplastic small round cell tumor patient-derived xenograft. Dis Model Mech 16:N/A (2023). PubMed: 37158111
- Yi JM et al. Human Neuralized is a novel tumour suppressor targeting Wnt/β-catenin signalling in colon cancer. EMBO Rep 24:e56335 (2023). PubMed: 37341560
- Murad HA & Alqurashi TM A novel nano-formulation of olmesartan medoxomil with improved delivery and efficacy in the treatment of indomethacin-induced duodenitis in rats. Braz J Med Biol Res 56:e12665 (2023). PubMed: 37255094
- Krieg S et al. Nuclear survivin is a prognosticator in gastroenteropancreatic neuroendocrine neoplasms: a meta-analysis. J Cancer Res Clin Oncol 148:2235-2246 (2022). PubMed: 35428913
- Udoh US et al. Tumor-Suppressor Role of the α1-Na/K-ATPase Signalosome in NASH Related Hepatocellular Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 35806364