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AB234257

重组Anti-Surfactant蛋白D/SP-D抗体[EPR21928-209] - BSA and Azide free

Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free

  • BOND RX™ Validated
  • RabMAb
  • Advanced Validation
  • Recombinant
  • 了解详情

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Rabbit Recombinant Monoclonal Surfactant protein D/SP-D antibody. Carrier free. Suitable for IP, WB, IHC-P, mIHC and reacts with Human, Recombinant fragment samples.

查看别名

COLEC7, PSPD, SFTP4, SFTPD, Pulmonary surfactant-associated protein D, PSP-D, SP-D, Collectin-7, Lung surfactant protein D

4 Images
Multiplex immunohistochemistry - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue. Panel A : Merged staining of anti-Podoplanin / gp36 (magenta; Opal™690), anti-Surfactant protein D/SP-D (green; Opal™520) and anti-Sialoadhesin/CD169 (red; Opal™570) on human lung. Panel B : Anti-Podoplanin / gp36 stained on alveolar type I cells. Panel C : Anti-Surfactant protein D/SP-D stained on alveolar type II cells. Panel D : Anti-Sialoadhesin/CD169 stained on macrophages. The section was incubated in three rounds of staining : in the order of ab236529 and ab234257 for 30 mins, then ab245735 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Surfactant protein D/SP-D with ab220423 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on alveolar type II cells of human lung (PMID : 10820266) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220423).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)

Negative control : No staining on human skeletal muscle (PMID : 10640760) is observed

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Surfactant protein D/SP-D with ab220423 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220423).

Immunoprecipitation - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)
  • IP

Supplier Data

Immunoprecipitation - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] - BSA and Azide free (AB234257)

Surfactant protein D/SP-D was immunoprecipitated from 0.35 mg human fetal lung lysate with ab220423 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220423 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1 : Human fetal lung lysate 10 μg (Input).
Lane 2 : ab220423 IP in human fetal lung lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220423 in human fetal lung lysate.
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

The molecular mass observed is consistent with what has been described in the literature (PMID : 9751757).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220423).

All lanes:

Immunoprecipitation - Anti-Surfactant protein D/SP-D antibody [EPR21928-209] (<a href='/products/primary-antibodies/surfactant-protein-d-sp-d-antibody-epr21928-209-ab220423'>ab220423</a>)

Predicted band size: 38 kDa

Observed band size: 43 kDa

false

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-Surfactant protein D/SP-D antibody [EPR21928-209]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21928-209

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, mIHC, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/1000", "mIHC-species-notes": "<p></p>" }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

ab234257 is the carrier-free version of ab220423.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Surfactant protein D (SP-D) also known as protein D functions as an important component of the innate immune system. It weighs around 43 kDa and is primarily expressed in pulmonary alveoli where lung cells synthesize it. SP-D belongs to the collectin family and has a C-type lectin domain that allows binding to carbohydrate structures on microbial surfaces. This ability enables SP-D to interact directly with various pathogens and infected cells targeting them for clearance. Researchers often study SP-D with 'anti-surfactant' antibodies using methods like the ELISA protocol including versions like ELISA Sp and ELISA Denise.
Biological function summary

SP-D contributes to host defense by enhancing the uptake and elimination of pathogens by immune cells such as macrophages. Part of a larger protein complex SP-D can opsonize bacteria and viruses facilitating phagocytosis. Its role extends to modulating inflammation by affecting the production of pro-inflammatory cytokines. By maintaining homeostasis in surfactant composition it indirectly supports lung function and protects against lung injury. SP-D’s function involves communication and transmission of signals within the immune system making it integral to pulmonary immune processes.

Pathways

Surfactant protein D is involved in the regulation of the complement and coagulation cascades as well as the pathogen recognition pathway. It is closely related to other collectins such as surfactant protein A (SP-A) which share similar mechanisms in the immune response. SP-D's interaction within these pathways highlights its role in identifying and neutralizing pathogens. Through its connections with related proteins SP-D plays an important part in modulating immune responses ensuring effective pathogen elimination without excessive inflammation.

SP-D has been linked with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. These conditions may involve impaired SP-D function or expression levels potentially due to genetic factors or environmental influences. SP-D also interacts with proteins like SP-A in these diseases participating in lung immune responses. Disrupted regulation of SP-D can contribute to chronic inflammatory states in the lungs exacerbating disease progression and leading to compromised lung function. Understanding SP-D's role in these contexts offers potential therapeutic insights for targeting pathological inflammation.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
See full target information SFTPD
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详情请参阅我们的条款与条件。
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