SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2] (ab72569)
![Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)](https://www.abcam.cn/ps/products/72/ab72569/Images/ab72569-473900-72569Flowdata-003.jpg "Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)")
Key features and details
- SureLight® APC Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: FRET, Flow Cyt (Intra)
- Reacts with: Species independent
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG1
概述
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产品名称
SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2]
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗 -
描述
SureLight® APC小鼠单克隆抗体[M2] to DDDDK tag (Binds to FLAG® tag sequence) -
宿主
Mouse -
偶联物
SureLight® APC. Ex: 652nm, Em: 657nm -
经测试应用
适用于: FRET, Flow Cyt (Intra)more details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
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常规说明
This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays.
Excitation maximum:652 nm Emission maximum:657.5 nm F:P 1:1.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose -
Concentration information loading... -
纯度
Size exclusion -
纯化说明
Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials. -
Primary antibody说明
This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays. -
克隆
单克隆 -
克隆编号
M2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
- DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
- DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
- PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
- Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
- PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
- Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72569于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| FRET |
Use at an assay dependent concentration.
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
FRET
Use at an assay dependent concentration. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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相关性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
别名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
图片
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Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
Flow Cytometry analysis of transfected HEK293 cells. Fixed and permeabilized cells stained with 1 µg/ml of ab72569
- Shaded histogram – HEK293 cells, expressing DYKDDDDK-tagged protein
- Open histogram – mock transfected control HEK293 cellsNo exactly the same info
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab72569 被引用在 7 文献中.
- Borges JP et al. Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. Elife 11:N/A (2022). PubMed: 36468682
- Edelstein HI et al. Elucidation and refinement of synthetic receptor mechanisms. Synth Biol (Oxf) 5:ysaa017 (2020). PubMed: 33392392
- Hayashi Y et al. Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels. J Biol Chem 293:17505-17522 (2018). PubMed: 30242129
- Roy G et al. A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies. MAbs 10:416-430 (2018). PubMed: 29400603
- Hayashi Y et al. Carboxyl-terminal Tail-mediated Homodimerizations of Sphingomyelin Synthases Are Responsible for Efficient Export from the Endoplasmic Reticulum. J Biol Chem 292:1122-1141 (2017). PubMed: 27927984
- Kerr SA et al. Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-Offs in New World Arenaviruses. J Virol 89:11643-53 (2015). PubMed: 26355089
- Demogines A et al. Dual host-virus arms races shape an essential housekeeping protein. PLoS Biol 11:e1001571 (2013). Flow Cyt . PubMed: 23723737