重组Anti-SUPT4H抗体[EPR6100(2)] (ab129187)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6100(2)] to SUPT4H
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SUPT4H抗体[EPR6100(2)]
参阅全部 SUPT4H 一抗 -
描述
兔单克隆抗体[EPR6100(2)] to SUPT4H -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human SUPT4H aa 50-150.
Database link: P63272 -
阳性对照
- 293T and MCF-7 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数(KD)
KD = 1.69 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6100(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129187于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa. |
靶标
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功能
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the SPT4 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6827 Human
- Entrez Gene: 20922 Mouse
- Entrez Gene: 287608 Rat
- Omim: 603555 Human
- SwissProt: P63272 Human
- SwissProt: P63271 Mouse
- SwissProt: B5DEM7 Rat
- Unigene: 439481 Human
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别名
- DRB sensitivity-inducing factor 14 kDa subunit antibody
- DRB sensitivity-inducing factor small subunit antibody
- DSIF p14 antibody
see all
图片
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All lanes : Anti-SUPT4H antibody [EPR6100(2)] (ab129187) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 13 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab129187 尚未被引用在任何文献中。