Anti-Superoxide Dismutase 3/EC-SOD抗体(ab21974)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Superoxide Dismutase 3/EC-SOD抗体
参阅全部 Superoxide Dismutase 3/EC-SOD 一抗 -
描述
兔多克隆抗体to Superoxide Dismutase 3/EC-SOD -
宿主
Rabbit -
特异性
Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT. -
经测试应用
适用于: IHC-P, IHC-Fr, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Superoxide Dismutase 3/EC-SOD (N terminal).
Sequence:WTGEDSAEPNSDSAEWIRD
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阳性对照
- IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21974于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| IHC-Fr |
1/200 - 1/2000.
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| WB | (1) |
1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa).
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| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
1/200 - 1/2000. |
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WB
1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa). |
靶标
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功能
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
组织特异性
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
序列相似性
Belongs to the Cu-Zn superoxide dismutase family. -
细胞定位
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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数据库链接
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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别名
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
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Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)ab21974 (1/1000) staining of human kidney tubule cells. -
Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974) at 1/500 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsSuperoxide Dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab21974 被引用在 12 文献中.
- Alaidaroos NYA et al. Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence. Stem Cell Res Ther 12:142 (2021). PubMed: 33596998
- Peng KT et al. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis. PLoS One 13:e0202501 (2018). PubMed: 30125327
- Shrestha P et al. Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells. Mol Cells 39:242-9 (2016). WB . PubMed: 26912083
- Cunningham MW et al. Pregnant rats treated with a high-fat/prooxidant Western diet with ANG II and TNF-a are resistant to elevations in blood pressure and renal oxidative stress. Am J Physiol Regul Integr Comp Physiol 308:R945-56 (2015). PubMed: 25810384
- Duscher D et al. Aging disrupts cell subpopulation dynamics and diminishes the function of mesenchymal stem cells. Sci Rep 4:7144 (2014). IHC-Fr ; Mouse . PubMed: 25413454