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Anti-Superoxide Dismutase 3/EC-SOD抗体[4G11G6] (ab80946)

Reviews (1)Q&A (4)References (15)
Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
  • Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)

Key features and details

  • Mouse monoclonal [4G11G6] to Superoxide Dismutase 3/EC-SOD
  • Suitable for: Flow Cyt, ELISA, ICC/IF, IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Superoxide Dismutase 3/EC-SOD抗体[4G11G6]
    参阅全部 Superoxide Dismutase 3/EC-SOD 一抗

  • 描述

    小鼠单克隆抗体[4G11G6] to Superoxide Dismutase 3/EC-SOD

  • 宿主

    Mouse

  • 特异性

    Detects extracellular Superoxide Dismutase 3/EC-SOD.

  • 经测试应用

    适用于: Flow Cyt, ELISA, ICC/IF, IHC-Fr, WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Full length native protein (purified) corresponding to Human Superoxide Dismutase 3/EC-SOD.
    Database link: P08294

  • 阳性对照

    • Human cartilage lysate

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab80946于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB (1)
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/100.
说明
Flow Cyt
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/100.

靶标

  • 功能

    Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

  • 组织特异性

    Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.

  • 序列相似性

    Belongs to the Cu-Zn superoxide dismutase family.

  • 细胞定位

    Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide Dismutase 3 antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

图片

  • Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
    Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
    Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?
    Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)

    ab80946 at 1/100 dilution staining Superoxide Dismutase 3/EC-SOD in human cartilage by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP-conjugated anti mouse secondary was used at 1/10 dilution. Image 'a' repersents control, 'b' staining of tissue from young human cartilage and 'c' show staining cartilage of a person suffered from osteoarthritis. 

  • Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
    Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
    Overlay histogram showing HEK293 cells stained with ab80946 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80946, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献 (15)

发表研究结果有使用 ab80946？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab80946 被引用在 15 文献中.

  • Lei Y  et al. Sequential inspiratory muscle exercise-noninvasive positive pressure ventilation alleviates oxidative stress in COPD by mediating SOCS5/JAK2/STAT3 pathway. BMC Pulm Med 23:385 (2023). PubMed: 37828534
  • Alferiev IS  et al. Stent-Based Gene Delivery for Coronary Disease. Methods Mol Biol 2573:217-233 (2022). PubMed: 36040598
  • Jang A  et al. Choroid plexus-CSF-targeted antioxidant therapy protects the brain from toxicity of cancer chemotherapy. Neuron 110:3288-3301.e8 (2022). PubMed: 36070751
  • Xu D  et al. Dexmedetomidine provides protection to neurons against OGD/R-induced oxidative stress and neuronal apoptosis. Toxicol Mech Methods 31:374-382 (2021). PubMed: 33648426
  • Gomes-Santos IL  et al. Exercise Training Preserves Myocardial Strain and Improves Exercise Tolerance in Doxorubicin-Induced Cardiotoxicity. Front Cardiovasc Med 8:605993 (2021). PubMed: 33869297
View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Takujiro Homma

Verified customer

提交于 Aug 02 2019

Question

Hi,
Thank you very much for your refund. Here is the image for the ab81154. I used 1:25 dilution. Because 1:1000 and 1:100 i can not see anything. You can see that in the KO rat, there is still a clear band. Also, the molecular weight for ECSOD should be around 27. But this band is aroudn 34KD. So I suspect this is a non-specific band. If you can contact to the lab who make this antibody, could you ask them to send me any data they had when they tested the antibody? Did they have negative or positive control for this antibody? Thank you very much.
Best,

Read More
Answer

Thank you very much for your email and for sending the additional image and information.

My colleague Elena has been out of the office, so I've sent your image on to the production laboratory and also requested the testing data from them. I apologize for the delay in getting back to you. This antibody was only tested in Western blotby the lab on the recombinant mouse protein, so there were no negative controls used for testing. Based on data shown in one of our online Abreviews, another user found a band around 26 kDa in mouse endothelial cell lysate, however negative control data is not shown-

https://www.abcam.com/Superoxide-Dismutase-3EC-SOD-antibody-C-terminal-ab81154.html&tab=abreviews&intabreviewid=30977

Unfortunately the lab can not send the data from their testing, and I am sorry for this lack of information. We will be investigating the problems with this antibody.

If you have any further questions, comments, or if there is anything else that we can do for you, please let me know and I'll be happy to help. Have a nice day.

Read More

Question

I will try blocking overnight at 4 degrees with 5% milk in TBST (let me know if you guys recommend a different blocking solution) with the antibody I saved in the fridge. Normally, I incubate with the antibody overnight (it ends up being around 17 hours), so to make sure timing goes ok, I plan on blocking overnight with milk for 24 hrs, and then doing the incubation in antibody for the normal ˜17 hours.

Read More
Answer

Blocking in milk should be suitable.

Read More

Question

-I have used this antibody successful in Wistar rat (chronic hypoxia and normoxic as control) pulmonary artery tissue for WB where the band appeared around 25 kDa. When I repeated the WB with isolated cells grown from the pulmonary arteries, about 10 bands appeared, with bands appearing at both 35 and 25 where SOD3 can be. Thus, we cannot successfully determine which band is actually SOD3. In addition, the band at 25 looks like a doublet, so we are unsure what this indicates as the pamplet does not mention a doublet showing up.

Read More
Answer

Thank you for contacting us.

I am sorry you are experiencing difficulty with this product. Since you obtained good results with the pulmonary artery tissue, it seems that maybe the isolation process is causing some extra bands. Can you give me some mroe details about this isolation? Our datasheet does show a faint band right above the 25 kD band. Increasing the blocking time to overnight at 4 degrees may resuce some of this background.Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

I purchased ab81154 and saw non-specific bands in my KO mouse. I was issued a replacement with ab80946 but I'm not seeing any bands in my positive control. I would like a refund.

Read More
Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. The amount paid for the antibody will be refunded to your credit card. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

Please do send along any data you have for ab81154 so we can forward it to the lab.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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