AB289821

重组Anti-Superoxide Dismutase 3/EC-SOD抗体[EPR25619-120] - BSA and Azide free (Detector)

Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR25619-120] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Superoxide Dismutase 3/EC-SOD antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

1 Images

Sandwich ELISA - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR25619-120] - BSA and Azide free (Detector) (AB289821)

sELISA

Lab

Sandwich ELISA - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR25619-120] - BSA and Azide free (Detector) (AB289821)

Sandwich ELISA of ab289805 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25619-120

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab289821 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Superoxide Dismutase 3/EC-SOD. The recommended pair for sandwich ELISA is:
Capture: ab289820, Human Superoxide Dismutase 3/EC-SOD Capture Antibody (unconjugated)
Detector: ab289821, Human Superoxide Dismutase 3/EC-SOD Detector Antibody (unconjugated)
The reference range value is 39.1-2500 pg/mL.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.

Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

See full target information Extracellular superoxide dismutase [Cu-Zn]

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Superoxide Dismutase 3/EC-SOD抗体[EPR25619-120] - BSA and Azide free (Detector)

Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR25619-120] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR25619-120] - BSA and Azide free (Detector) (AB289821)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原